On-demand workflow design for image processing and analysis

Quantitative measurement of intracellular forces on living cells, but also within tissues (Drosophila, pupae, mouse intestines). Mechanotransduction, micro-nanorheology. High-resolution fluorescence imaging using remote focusing coupled with force measurements.
Compression force measurements

STED correlative imaging with molecular dynamic and interaction (FCS-FLIM-FRAP-FRET) on thin, thick samples

Light-sheet imaging of fixed and live samples. We can afford (cells, IPS, organoïds, hydra, fish, human tissues) because the microscope is installed in Biology Safety Level 2

Low signal fast live imaging at nano-scale level (Airy / Fast Airyscan , Live-3D-STED, Live-3D-SLMN)

Cell-biology expertise: providing cells(epithelial, glial, neuronal) and organoïds, labelling (transfections or probe-Test), and imaging for physicist, chemist that would like to test a prototype. Including : Guidance on super-resolution modalities (collaboration) : from system choice (SIM-STED-STORM) to images processing and data analysis (tools development).

Full pipeline support on light sheet and biphoton imaging (from sample prep and tissue clearing to imaging)

Full pipeline support for single molecule RNA FISH

On demand from basic training for image acquisition to full pipeline support in multi modal imaging

Forces (nN range) are applied or probed by atomic force microscopy, while samples are correlatively and simultaneously observed by fluorescence microscopy in time-resolved confocal microscopy, epifluorescence or TIRF. Imaging can be coupled to single molecule localisation superresolution microscopy