Cellular imaging for neurodegenerative diseases studies : nervous system, neurons in culture, cell biology, cytoskeleton

2D or 3D electron microscopy morphometric analysis of the central nervous system with Deep FIB-SEM and IA-based image analysis

nm-characterization of multitypes materials like liposomes, emulsion, hydrogel and porous materials by Cryo-TEM/SEM, and electron tomography,

Connecting different resolutions for correlative confocal/SEM and confocal/TEM on adherent cells

We combine latest developments in cryo sample preparation and imaging by electron microscopies (cryo-FIB/SEM and cryo-TEM) to observe biomolecules of interest (ribosomes, carboxysomes, viruses, nuclear pore complexes…) within their cellular or tissular environment. Using cryo-electron tomography followed by image analysis, we are able to determine their 3D structures with near atomic resolution. Altogether, these methodologies pave the way for in situ structural biology studies.

Organoids (lung, endometriosis, digestive tract, adipose tissue, bone marrow, skin, bladder, fallopian tube): integrative analysis (sample preparation, acquisition – light sheet, confocal, two-photon, high-content screening, multiparametric analysis, 3D imaging, analyses, flow cytometry and imaging)

Expertise in preparation and morphological analysis using TEM (brain and spinal cord)

Full workflow support on surface morphology and ultrastructural analysis in SEM and TEM

Single-particle analyses performed using TEM after negative staining

Serial sectioning, SBF, FIB-SEM

Correlative light/electron microscopy (CLEM)

3D cellular or tissue imaging by electron microscopy (SerialBlock Face SEM and thick sections in electron tomography)