We offer workflows that enables tissular to subcellular analysis with a specialization in in immune tissues. Our team provides guidance and support at key stages throughout the process. We offer different organs fixation and sectioning methods including cryo-fixed or chemically fixed sample. Accordingly we offer various types of immunolabeling techniques using on these sections. We provide imaging services that allows to get a stitched confocal image of large samples using a high-speed confocal imaging system. Ultimately, we offer a wide range of tools enabling image restauration and analysis at the cellular and subcellular like surface quantification, intensity measurement, structures counting.
To support this workflow we rely on a fully equipped preparation laboratory and two microscopes which can be used in wide field or confocal modes using a CSU X1 Yokogawa confocal head on a Zeiss Axio Observer Z1. We have 2 computers with GPU and various analysis tools: conventional tools like Imaris, ImageJ/FIJI, Qupath, and IA tools like Ilastik, Cellpose, DeepMIB and different conda environments for Deep learning applications.