Maintain of the mouse living organs under 2P microscope Bruker 2P+, thanks to perfusion and oxygenated medium

Bruker 2P+, Discovery laser (680 to 1340 nm), mouse anesthesy and stabulation. Cleared mouse organs (gut, lymph nodes..) and 2P microscopy

Intravital imaging (murin or tissue of human origin) BSL-2 multi-photon spectral FRET-FLIM, SHG THG (collagen I & III, myosin)

Ultrafast in vivo functional optical imaging using AOD two-photon microscopy (brain slices and small animals)

Inscoper module for control, Nikon FNE stand, Yokogawa CSU W1 head, two cameras

Ultima 2Plus Bruker, Chameleon vision II
Second laser 1000-1300 nm.

In vivo visualization of plant tissues cellular development, as well as of their response to climate variations
(Key-words : PICPLANT facility with greenhouses (S2), 3 growth chambers for small and large plants, CONVIRON climatic chamber, histology, stereomicroscope, Spectral-FLIM confocal microscope.

Mono and bi-photon longitudinal imaging with flexible windows and/or stabilizing chambers for imaging peripheral organs (e.g. heart, lung, liver, gut, pancreas) and human organoids/spheroids/tumoroids implanted in mouse or zebrafish models. This provides information about how they get connected to the blood vasculature.

Longitudinal imaging with one/two colour miniscope with high speed acquisition. Pre-implantation of mouse models with GRIN lenses (objectives from 4 to 7.6mm long and 0.6 to 1.4mm diameter) in brain region expressing specific fluorescent reporters. Mice are imaged for days to several months and fluorescent signals can be correlated with cognitive, behaviour and physiological outcomes.

Intravital mono or bi-photon imaging with either short or long (up to 2 cm) working-distance objectives and coupled with cellular manipulation (mouse, fish and insect models). This can be applied to high resolution cellular imaging of molecule extravasation (e.g. nanobodies, drugs, hormones), capillary blood flow, cellular migration and signaling (e.g. calcium, ligand sensors), and molecule distribution into the extracellular space.

With the help of the CircuitPhotonics platform we can offer several approaches to imaging living organisms, on brain slices with a TriMscope 1 (LaVision Biotec), in mouse brains with several 2-photon excitation stations (and one with 3-photon excitation) Bruker Ultima 2, Karthala, Zeiss NLO …