Maintain of the mouse living organs under 2P microscope Bruker 2P+, thanks to perfusion and oxygenated medium

Correlation microscopy between super-resolution acquisition and collagen harmonic signalling

X-ray Microtomography of large samples (1mm up to 30cm in diameter)
(Key words : 3D volumetric acquisition; large samples; non-invasive inspection; tomodensitometry)

We use QPI to quantify the dry mass, intra-cellular transport, cell growth, cell migration

We use different imaging modalities to probe chemical fingerprinting in pharmalogical drug studies or cancer diagnosis. Many types of samples can be imaged such as liquids, powders, cells, tissues, organisms

We use multiple imaging systems to probe the polarization of fluorescent molecules. This gives an access to their molecular organization and orientation within cells and organisms.

Holotomography merges holography and tomography to solve the challenge of recovering light phase shifts to generate image contrast in Quantitative Phase Imaging. HT illuminates a cell with very low power visible light at various illumination angles and measures the phase delay of the transmitted light. Holotomography’s advantages lie in its capability to provide non-invasive, label-free 3D images of live cells, allowing real-time studies while the measurement of RI tomogram also allows quantitative analysis. Its data acquisition speed surpasses traditional methods, making it invaluable for quick results in medical diagnostics and drug research.

Unlabelled imaging can be used to characterise histological sections by mapping them without treatment. It is also possible to complete the analysis using immunohistochemistry, multiplex immunoflorescence or histological staining technologies. A holotomography Nanolive and a serial block face imaging (Kratoscope, based on image recording triggered with a sectioning device such as a microtomoe or cryoststat) are available on the MIcroPIcell facility

Imaging by Raman spectroscopy (RAMAN DXR2xi imager, ThermoFisher Scientific): each molecule has a unique spectral signature, determined by the Raman photons emitted after interaction with light. These photons are collected and analysed to provide information on the structure and chemical composition of the sample. We are mapping several thousand spectra on frozen tissue sections just a few microns thick.
Stimulated Raman imaging (SRS_microscope BondXplorer, Lightcore Technologies) complements spontaneous Raman spectroscopy. It can be used to target certain areas of interest in the spectrum in order to image chemical compounds. Acquisitions are therefore faster and can be carried out over larger fields of view.
Anti-Stokes Coherent Raman Scattering (CARS) microscopy provides high-resolution, high-speed, high-sensitivity, non-invasive imaging. It offers the possibility of visualising specific biomolecules without prior labelling.

In vivo imaging for neurosciences: labelled & label-free sample imaging
(Key-words: Two-photon imaging, SHG)