Photomanipulation of biological objects (photoablation on spinning disks and on confocal equipped with 2photons, optogenetics)

Quantifying Physical parameters (rigidity, tension force production, turgor pressure…) from animal/plant cell to tissue (TFM, Brillouin imaging, photoablation, Triboindenteur, AFM, AFM Hybrid Stage)

Gene expression, DNA damage, DNA structure and DNA-binding proteins structures, super-resolution (SMLM, RIM, SIM, Airyscan), single particle tracking, photo-manipulation, Correlative Light Electron Microscopy (CLEM)

Atomic force microscopy on living cells or 3D samples is a powerful technique to measure the mechanical properties such as elastic moduli , rigidity, adhesion force, adhesion energy. Combining optical images, AI and AFM, cell population’s mechanome becomes accessible. 3D samples (tissues, organoids) mechanical properties require specific expertise we also provide.

Quantitative measurement of intracellular forces on living cells, but also within tissues (Drosophila, pupae, mouse intestines). Mechanotransduction, micro-nanorheology. High-resolution fluorescence imaging using remote focusing coupled with force measurements.
Compression force measurements

Forces (nN range) are applied or probed by atomic force microscopy, while samples are correlatively and simultaneously observed by fluorescence microscopy in time-resolved confocal microscopy, epifluorescence or TIRF. Imaging can be coupled to single molecule localisation superresolution microscopy

The offer provides state-of-the-art instruments optimized for the detailed characterization of upconversion nanoparticles (UCNPs). These systems enable the study of their photophysics and stability, as well as their application in bioimaging. Particular emphasis is placed on long-term single-particle tracking in living cells under near-infrared excitation..

Depending on the speed and resolution need for your live imaging project , the IGBMC platform propose different imaging systems equipped with an environmental chamber. Wide-field triggered Zeiss AxioObserver system will allow long term analysis of cell motility, wound healing application, Long-term observation of multi-labelled living cells, intracellular dynamics processes analysis (Calcium, ratiometric FRET imaging, vesicles )…High spatial 3D resolution imaging of living cells or tissues is possible on 1 point scanning (Leica TCS SP8X) or on 2 spinning disk microscopes (Yokogawa CSU-X1 or CSU-W1 scanning head) for fast acquisitions with reduced photobleaching. Live-SR modules are available on our 2-spinning disk systems enabling live Super-resolution up to 105 nm at high speed with no specific fluorophores required. 1 TIRF system equipped with a EM-CCD camera allows super resolution imaging along the optical axis (~100 nm) so imaging of membrane processes, such as receptor-ligand interactions, endocytosis, viral infection, or cell adhesion to surfaces.
2 new systems will be installed at the IGBMC platform in Spring 2025, opening up new possibilities for live imaging : 1 Lattice lightsheet will allow dynamic imaging of full sample volumes (cells, spheroids, organoids, zebrafish/c-elegans/drosophilia embryos…) with high temporal and spatial resolution and next to no phototoxicity and bleaching; 1 lattice SIM system will allow cellular, subcellular, and even sub-organelle structures in living specimens imaging in 2D and 3D with high speed, resolution below the diffraction limit (120nm, 60nm with processing) , with no need of specific fluorophores. Highest super resolution live imaging also available at our neighbored FBI Alsace Node “Plateforme Imagerie In Vitro” at INCI with the Leica Steallris STED with TauSTED and Falcon lifetime imaging option.

Several modules or lasers are available for specific photomanipulation experiments at the sub-cellular level: high energy 355 nm pulsed lasers allow uncageaging, DNA damage induction, or precise photoablation (available on a TCS SP8 confocal microscope or on fast spinning disk with Yokogawa CSU X1 scanning head), laser microdissection system, Multiphoton confocal system also available for localized damage or precise ablation, FRAP module available on 2 confocal systems and on 2 spinning disk (equipped with liveSR module), allowing the analysis of intracellular dynamics, controlled photoactivation or photoconversion applications.
Following acquisition, solution or assistance for quantitative data analysis, curve fitting and kinetics measurements can be provided.