Imaging, quantifiying and controlling cell metabolism (FLIM, TIRF-FastFLIM, ratipmetric-SD, optogenetis…)

Multiscale measurements of molecular dynamics in cell and tissue (SPT, FCS, tICS, AiryScan, SIM, STED, SMLM, , Live SR, FRAP, live confocal imaging, Live TIRF and optogenetics)

Quantitative FRET and stoichiometry measurements to map biological activity in cells : fluorescence quantification via an universal QuanTI-FRET protocol

STED correlative imaging with molecular dynamic and interaction (FCS-FLIM-FRAP-FRET) on thin, thick samples

Functional microscopy at the molecular, cellular, and tissue levels (FLIM-FCS, FRET, FRAP, laser ablation)

On demand from basic training for image acquisition to full pipeline support in multi modal imaging

IX83 microscope equipped with two PENTA SET TIRF, detector (sCMOS ORCA Fusion BT), TIRF module (445nm, 488nm, 514nm, 561nm, 640nm), cellVivo module, cellSens software (Evident)

Multidirectional illumination for transparent living samples
(Alpha3 facility edition Phase View) and XXL chamber for transparent samples (Ultramicroscope Blaze, Milteniy BioTech)

Live Imaging and molecular dynamics for cell, organoids, tissue and embryo : from sample preparation with access to a L2 cell culture room, to microscopy and data analysis
(Key words : spinning disk, lattice light sheet, widefield, LightSheet, FRAP, photoconversion, FCS, spot variation FCS, TIRF, single molecule, FRET, FLIM))

Single Molecule mRNA Imaging : smFISH in 96 plates and low-troughput multiplexed smFISH in collaboration with Edouard Bertrand’ lab, from probe design and handling to data pre-processing and training for analysis.
(Keywords : smFISH, multiplexed FISH, HCS microscope, Microfluidics coupled to widefield and STORM microscope)

Measuring biomolecular structure, dynamics and interactions in vitro and in living cells. This includes distances and interactions measurements by single molecule FRET and FRET/FLIM and interactions and dynamics by FCS