Zeiss CD7, XY, Z-stacks, multi-points, mosaics, time-lapse

The automation of fluorescence microscopy represents a challenge for the study of rare or transient events in biology and medicine. It relies on intelligent devices that integrate and interpret observed data and react to targeted biological events. The Roboscope (MRIC facility) is a new autonomous microscope combining interruption of the acquisition flow and event detection by deep learning. This system is distinguished by the parallelization of tasks, simultaneously performing image acquisition and data analysis to accelerate event-driven acquisition.
Nikon confocal microscopes (MicroPICell facility) enable adaptive feedback mmicroscopy, a form of smart microscopy where image analysis is performed during acquisition, and the results are used to automatically adjust microscope settings or guide the experiment in real time. By integrating user-designed advanced analysis workflows directly into the acquisition process, this approach makes it possible to detect rare or highly specific events and automatically adjust the microscope settings in real time.

Our 2 platforms offer routine services in histology, tissue processing, thin sections, staining, immunohistochemistry and digitisation of microscopy slides (Hamamtsu S360 & S60 scanners).

High content microscopy with Nikon’s confocal LIPSI system combines automated image acquisition with integrated analysis, making it particularly powerful for high-content screening and live-cell studies. By reducing manual steps such as focusing, exposure adjustment, and data processing, it ensures reproducibility and standardization across experiments. This system is equipped with a robotic arm for scanning up to 20 multi-well plates or 80 slides on both fixed and live samples. A colour camera is also available for combining fluorescence acquisition with histological staining.

Depending on the speed and resolution need for your live imaging project , the IGBMC platform propose different imaging systems equipped with an environmental chamber. Wide-field triggered Zeiss AxioObserver system will allow long term analysis of cell motility, wound healing application, Long-term observation of multi-labelled living cells, intracellular dynamics processes analysis (Calcium, ratiometric FRET imaging, vesicles )…High spatial 3D resolution imaging of living cells or tissues is possible on 1 point scanning (Leica TCS SP8X) or on 2 spinning disk microscopes (Yokogawa CSU-X1 or CSU-W1 scanning head) for fast acquisitions with reduced photobleaching. Live-SR modules are available on our 2-spinning disk systems enabling live Super-resolution up to 105 nm at high speed with no specific fluorophores required. 1 TIRF system equipped with a EM-CCD camera allows super resolution imaging along the optical axis (~100 nm) so imaging of membrane processes, such as receptor-ligand interactions, endocytosis, viral infection, or cell adhesion to surfaces.
2 new systems will be installed at the IGBMC platform in Spring 2025, opening up new possibilities for live imaging : 1 Lattice lightsheet will allow dynamic imaging of full sample volumes (cells, spheroids, organoids, zebrafish/c-elegans/drosophilia embryos…) with high temporal and spatial resolution and next to no phototoxicity and bleaching; 1 lattice SIM system will allow cellular, subcellular, and even sub-organelle structures in living specimens imaging in 2D and 3D with high speed, resolution below the diffraction limit (120nm, 60nm with processing) , with no need of specific fluorophores. Highest super resolution live imaging also available at our neighbored FBI Alsace Node “Plateforme Imagerie In Vitro” at INCI with the Leica Steallris STED with TauSTED and Falcon lifetime imaging option.

Several modules or lasers are available for specific photomanipulation experiments at the sub-cellular level: high energy 355 nm pulsed lasers allow uncageaging, DNA damage induction, or precise photoablation (available on a TCS SP8 confocal microscope or on fast spinning disk with Yokogawa CSU X1 scanning head), laser microdissection system, Multiphoton confocal system also available for localized damage or precise ablation, FRAP module available on 2 confocal systems and on 2 spinning disk (equipped with liveSR module), allowing the analysis of intracellular dynamics, controlled photoactivation or photoconversion applications.
Following acquisition, solution or assistance for quantitative data analysis, curve fitting and kinetics measurements can be provided.

The Imaging Facility at IBMP-CNRS can help researchers design, adapt, build or automate original pieces of laboratory equipment in the field of biological imaging.

The Imaging Facility at IBMP-CNRS provides researchers with access to state-of-the-art microscopy techniques, including fluorescence, confocal, and 3D scanning electron microscopy. The platform supports a variety of sample preparation workflows, such as live-cell imaging and cryotechniques, and offers guidance at every step, from experimental design to image data analysis. Services are available for projects on plant tissues, microbial systems, and cell cultures, enabling users to visualize structures and processes at high resolution. The facility is dedicated to making advanced imaging approaches accessible, fostering collaboration, and helping researchers obtain reliable, quantitative results.