Inflammatory and cardiovascular diseases are complex, multifactorial conditions that affect multiple organs and cellular components. To tackle these intricate biological challenges, we employ and advance multi-modal and multi-scale imaging and flow cytometry pipelines. These pipelines include the entire process from sample preparation, image acquisition to image analysis and quantification. Our workflows are adpated to various tissues, including the heart, kidneys, blood vessels, muscles, liver and adipose tissue. They operate across scales ranging from whole organs to subcellular levels, employing techniques such as widefield imaging, confocal spectral imaging, high-resolution imaging (Airyscan), high-content imaging (Axioscan 7, Opera Phenix), and cytometry-based analysis (spectral, sorting, and analysis).
Organ: Heart
Workflow including sample preparation, light sheet microscopy, confocal microscopy, intravital microscopy, and image analysis.
Inscoper module for control, Nikon FNE stand, Yokogawa CSU W1 head, two cameras
Multidirectional illumination for transparent living samples
(Alpha3 facility edition Phase View) and XXL chamber for transparent samples (Ultramicroscope Blaze, Milteniy BioTech)
Histology : from sample preparation with the RHEM and PHIV facility (human, animal and plant tissue) to digitisation of virtual slides and image analysis
(Key words : brightfield and multiplex staining, Nanozoomer (brightfield), Axioscan (fluorescence), Pannoramic (brightfield and fluorescence), QuPath and Histometrix with machine/deep learning)
With the help of the IBDM Lignt Microscopy Facility we can offer an comprehensive CLEM Pipeline from live fluorescence imaging to EM imaging (in 2D and 3D)
As Volume EM techniques we offer Electron Tomography and Serial Blockface Scanning Electron Microscopy. The targeting of a precise ROI can be achieved by integrating Light Microscopy or X-ray imaging in the pipeline.
We work on mouse models (embryos at different stages, mouse organs, tumor samples) with solvent based clearing methods (iDisco modified, home made protocols depending on the samples).
Comprehensive quantitative analysis pipeline for muscle biopsies, integrating advanced imaging and artificial intelligence tools to extract precise morphological and functional parameters.
Ultrastructural studies in transmission electron microscopies (2D -3D) for plant sciences and animals sciences: from sample preparation (conventional and cryogenic conditions), to imaging & data processing
(Key-words: Tokuyasu, Immunolabelling, TEM, SBF, tomography, 3D reconstructions, segmentation, image analysis)
Scanning electron microscopy in cryogenic conditions for plant imaging and soft materials: from sample preparation, to imaging & data processing (Key-words: HPF, plunge freezing, cryofracture, cryoSEM, cryoSEM EDS)
Volumetric electron microscopy for cancer, neurosciences, plants sciences: from sample preparation (conventional and cryogenic conditions), to imaging & data processing (Key-words: HPF, FS, SBF, Array-tomography, FIB SEM, cryoFIB SEM, µCT)