Organoids (lung, endometriosis, digestive tract, adipose tissue, bone marrow, skin, bladder, fallopian tube): integrative analysis (sample preparation, acquisition – light sheet, confocal, two-photon, high-content screening, multiparametric analysis, 3D imaging, analyses, flow cytometry and imaging)

Multidirectional illumination for transparent living samples
(Alpha3 facility edition Phase View) and XXL chamber for transparent samples (Ultramicroscope Blaze, Milteniy BioTech)

Histology : from sample preparation with the RHEM and PHIV facility (human, animal and plant tissue) to digitisation of virtual slides and image analysis
(Key words : brightfield and multiplex staining, Nanozoomer (brightfield), Axioscan (fluorescence), Pannoramic (brightfield and fluorescence), QuPath and Histometrix with machine/deep learning)

With the help of the IBDM Lignt Microscopy Facility we can offer an comprehensive CLEM Pipeline from live fluorescence imaging to EM imaging (in 2D and 3D)

As Volume EM techniques we offer Electron Tomography and Serial Blockface Scanning Electron Microscopy. The targeting of a precise ROI can be achieved by integrating Light Microscopy or X-ray imaging in the pipeline.

High speed, low photobleaching with sub cellular resolution and high detection sensitivity for cleared whole organs, brains or small embryos is offered by the Zeiss light Sheet LS7 -Multiphoton confocal system also available depending on specific applications (including Second Harmonic Generation), large field of view spinning disk (Leica microscope equipped with Yokogawa CSU-WI scanning head) also available and can be used for living sample. Both multiphoton confocal and spinning disk are equipped with 20x, 0.95 NA Water IMM objective providing 2.2 mm free working distance allowing in depth imaging with an excellent 3D resolution in tissue, small animal models, organs, organoids…
Following acquisition, solution or assistance for 3D image analysis, segmentation and quantification can be provided using dedicated software (Imaris, Cellpose, Napari, specific Python scripts…) For this purpose, the facility is equipped with several GPU workstations for efficient processing of big data, available to users.