On-demand workflow design for image processing and analysis

Phytotronic cabinets and expression of fluorescent fusion proteins/tags (transformation by electroporation)

Workflow including sample preparation, 2P microscopy, confocal microscopy, light sheet microscopy, ophthalmic imaging/OCT/ERG and image analysis.

Zeiss CD7, XY, Z-stacks, multi-points, mosaics, time-lapse

Volumetric electron microscopy : from sample preparation (chemical fixation), to imaging & data processing
(Key-words: SBF, Array-tomography, µCT, micro-wave, nanotomy)

Live Imaging and molecular dynamics for cell, organoids, tissue and embryo : from sample preparation with access to a L2 cell culture room, to microscopy and data analysis
(Key words : spinning disk, lattice light sheet, widefield, LightSheet, FRAP, photoconversion, FCS, spot variation FCS, TIRF, single molecule, FRET, FLIM))

Histology : from sample preparation with the RHEM and PHIV facility (human, animal and plant tissue) to digitisation of virtual slides and image analysis
(Key words : brightfield and multiplex staining, Nanozoomer (brightfield), Axioscan (fluorescence), Pannoramic (brightfield and fluorescence), QuPath and Histometrix with machine/deep learning)

Super-resolution-based imaging for cell, tissue and embryo : from sample preparation, to imaging & data processing
(Key-words: Airyscan, STED, STORM, PALM, expansion microscopy alone or combined with other super-resolution technics, data analysis)

Longitudinal imaging with one/two colour miniscope with high speed acquisition. Pre-implantation of mouse models with GRIN lenses (objectives from 4 to 7.6mm long and 0.6 to 1.4mm diameter) in brain region expressing specific fluorescent reporters. Mice are imaged for days to several months and fluorescent signals can be correlated with cognitive, behaviour and physiological outcomes.

3D SIM on microorganisms