To tackle complex biological questions in aging and geroscience, we design and implement advanced multimodal, multiparametric, and multiscale pipelines in imaging, flow cytometry, and image flow cytometry. Our workflows span the entire process—from sample preparation to data acquisition and multidimensional analysis. We investigate biological systems across multiple scales, from subcellular structures such as mitochondria to organoids, whole organs, and even entire organisms. Our pipelines are tailored to a wide range of tissues, including adipose tissue, muscle, bone marrow, skin, blood, and periodontal tissue. In addition, our platforms provide access to complementary techniques such as second- and third-harmonic generation (SHG, THG), spectral confocal imaging, high-content imaging, image-based cytometry, conventional and spectral flow cytometry, and mechanobiological analysis. These integrative approaches are designed to support comparative and translational research across multiple species, including fish (with an emphasis on the African turquoise killifish as a model of aging), drosophila, mice, and humans.
Organ: Skin
Organoids (lung, endometriosis, digestive tract, adipose tissue, bone marrow, skin, bladder, fallopian tube): integrative analysis (sample preparation, acquisition – light sheet, confocal, two-photon, high-content screening, multiparametric analysis, 3D imaging, analyses, flow cytometry and imaging)
HPF, GP2, AFS2 Leica Microsystems
Histology : from sample preparation with the RHEM and PHIV facility (human, animal and plant tissue) to digitisation of virtual slides and image analysis
(Key words : brightfield and multiplex staining, Nanozoomer (brightfield), Axioscan (fluorescence), Pannoramic (brightfield and fluorescence), QuPath and Histometrix with machine/deep learning)
With the help of the IBDM Lignt Microscopy Facility we can offer an comprehensive CLEM Pipeline from live fluorescence imaging to EM imaging (in 2D and 3D)
As Volume EM techniques we offer Electron Tomography and Serial Blockface Scanning Electron Microscopy. The targeting of a precise ROI can be achieved by integrating Light Microscopy or X-ray imaging in the pipeline.
Ultrastructural studies in transmission electron microscopies (2D -3D) for plant sciences and animals sciences: from sample preparation (conventional and cryogenic conditions), to imaging & data processing
(Key-words: Tokuyasu, Immunolabelling, TEM, SBF, tomography, 3D reconstructions, segmentation, image analysis)
Scanning electron microscopy in cryogenic conditions for plant imaging and soft materials: from sample preparation, to imaging & data processing (Key-words: HPF, plunge freezing, cryofracture, cryoSEM, cryoSEM EDS)
Volumetric electron microscopy for cancer, neurosciences, plants sciences: from sample preparation (conventional and cryogenic conditions), to imaging & data processing (Key-words: HPF, FS, SBF, Array-tomography, FIB SEM, cryoFIB SEM, µCT)