On-demand workflow design for image processing and analysis

Organoids (lung, endometriosis, digestive tract, adipose tissue, bone marrow, skin, bladder, fallopian tube): integrative analysis (sample preparation, acquisition – light sheet, confocal, two-photon, high-content screening, multiparametric analysis, 3D imaging, analyses, flow cytometry and imaging)

STED correlative imaging with molecular dynamic and interaction (FCS-FLIM-FRAP-FRET) on thin, thick samples

Light-sheet imaging of fixed and live samples. We can afford (cells, IPS, organoïds, hydra, fish, human tissues) because the microscope is installed in Biology Safety Level 2

Low signal fast live imaging at nano-scale level (Airy / Fast Airyscan , Live-3D-STED, Live-3D-SLMN)

Cell-biology expertise: providing cells(epithelial, glial, neuronal) and organoïds, labelling (transfections or probe-Test), and imaging for physicist, chemist that would like to test a prototype. Including : Guidance on super-resolution modalities (collaboration) : from system choice (SIM-STED-STORM) to images processing and data analysis (tools development).

Full pipeline support on light sheet and biphoton imaging (from sample prep and tissue clearing to imaging)

On demand from basic training for image acquisition to full pipeline support in multi modal imaging

Workflow including sample preparation, 2P microscopy, confocal microscopy, light sheet microscopy, ophthalmic imaging/OCT/ERG and image analysis.

Multidirectional illumination for transparent living samples
(Alpha3 facility edition Phase View) and XXL chamber for transparent samples (Ultramicroscope Blaze, Milteniy BioTech)

X-ray Microtomography of large samples (1mm up to 30cm in diameter)
(Key words : 3D volumetric acquisition; large samples; non-invasive inspection; tomodensitometry)

Volumetric electron microscopy : from sample preparation (chemical fixation), to imaging & data processing
(Key-words: SBF, Array-tomography, µCT, micro-wave, nanotomy)