On-demand workflow design for image processing and analysis
Research organism: Explant organ
Maintain of the mouse living organs under 2P microscope Bruker 2P+, thanks to perfusion and oxygenated medium
STED correlative imaging with molecular dynamic and interaction (FCS-FLIM-FRAP-FRET) on thin, thick samples
Light-sheet imaging of fixed and live samples. We can afford (cells, IPS, organoïds, hydra, fish, human tissues) because the microscope is installed in Biology Safety Level 2
Low signal fast live imaging at nano-scale level (Airy / Fast Airyscan , Live-3D-STED, Live-3D-SLMN)
Cell-biology expertise: providing cells(epithelial, glial, neuronal) and organoïds, labelling (transfections or probe-Test), and imaging for physicist, chemist that would like to test a prototype. Including : Guidance on super-resolution modalities (collaboration) : from system choice (SIM-STED-STORM) to images processing and data analysis (tools development).
Full pipeline support on light sheet and biphoton imaging (from sample prep and tissue clearing to imaging)
On demand from basic training for image acquisition to full pipeline support in multi modal imaging
All equipment necessary for cell culture (PSM, incubators, centrifuges, etc.)
Workflow including sample preparation, 2P microscopy, confocal microscopy, light sheet microscopy, ophthalmic imaging/OCT/ERG and image analysis.
Multidirectional illumination for transparent living samples
(Alpha3 facility edition Phase View) and XXL chamber for transparent samples (Ultramicroscope Blaze, Milteniy BioTech)
Histology : from sample preparation with the RHEM and PHIV facility (human, animal and plant tissue) to digitisation of virtual slides and image analysis
(Key words : brightfield and multiplex staining, Nanozoomer (brightfield), Axioscan (fluorescence), Pannoramic (brightfield and fluorescence), QuPath and Histometrix with machine/deep learning)