On-demand workflow design for image processing and analysis

Bruker 2P+, Discovery laser (680 to 1340 nm), mouse anesthesy and stabulation. Cleared mouse organs (gut, lymph nodes..) and 2P microscopy

To tackle complex biological questions in aging and geroscience, we design and implement advanced multimodal, multiparametric, and multiscale pipelines in imaging, flow cytometry, and image flow cytometry. Our workflows span the entire process—from sample preparation to data acquisition and multidimensional analysis. We investigate biological systems across multiple scales, from subcellular structures such as mitochondria to organoids, whole organs, and even entire organisms. Our pipelines are tailored to a wide range of tissues, including adipose tissue, muscle, bone marrow, skin, blood, and periodontal tissue. In addition, our platforms provide access to complementary techniques such as second- and third-harmonic generation (SHG, THG), spectral confocal imaging, high-content imaging, image-based cytometry, conventional and spectral flow cytometry, and mechanobiological analysis. These integrative approaches are designed to support comparative and translational research across multiple species, including fish (with an emphasis on the African turquoise killifish as a model of aging), drosophila, mice, and humans.

Organoids (lung, endometriosis, digestive tract, adipose tissue, bone marrow, skin, bladder, fallopian tube): integrative analysis (sample preparation, acquisition – light sheet, confocal, two-photon, high-content screening, multiparametric analysis, 3D imaging, analyses, flow cytometry and imaging)

Ultrafast in vivo functional optical imaging using AOD two-photon microscopy (brain slices and small animals)

Correlation microscopy between super-resolution acquisition and collagen harmonic signalling

Super-resolution acquisition coupled to high-end atomic force microscopy

Full pipeline support on light sheet and biphoton imaging (from sample prep and tissue clearing to imaging)

Full pipeline support for single molecule RNA FISH

On demand from basic training for image acquisition to full pipeline support in multi modal imaging

Workflow including sample preparation, light sheet imaging, and fluorescence macroscopy