STED correlative imaging with molecular dynamic and interaction (FCS-FLIM-FRAP-FRET) on thin, thick samples

Light-sheet imaging of fixed and live samples. We can afford (cells, IPS, organoïds, hydra, fish, human tissues) because the microscope is installed in Biology Safety Level 2

Phytotronic cabinets and expression of fluorescent fusion proteins/tags (transformation by electroporation)

All equipment necessary for cell culture (PSM, incubators, centrifuges, etc.)

Workflow from sample preparation, MET and cryo-confocal, advanced photonic microscopy
WF/confocal/FLIM, CLEM, image analysis (AMIRA)

Talos F200X G2

HPF, GP2, AFS2 Leica Microsystems

X-ray Microtomography of large samples (1mm up to 30cm in diameter)
(Key words : 3D volumetric acquisition; large samples; non-invasive inspection; tomodensitometry)

Volumetric electron microscopy : from sample preparation (chemical fixation), to imaging & data processing
(Key-words: SBF, Array-tomography, µCT, micro-wave, nanotomy)

In vivo visualization of plant tissues cellular development, as well as of their response to climate variations
(Key-words : PICPLANT facility with greenhouses (S2), 3 growth chambers for small and large plants, CONVIRON climatic chamber, histology, stereomicroscope, Spectral-FLIM confocal microscope.