On-demand workflow design for image processing and analysis

Photomanipulation of biological objects (photoablation on spinning disks and on confocal equipped with 2photons, optogenetics)

Connecting different resolutions for correlative confocal/SEM and confocal/TEM on adherent cells

Imaging, quantifiying and controlling cell metabolism (FLIM, TIRF-FastFLIM, ratipmetric-SD, optogenetis…)

Multiscale measurements of molecular dynamics in cell and tissue (SPT, FCS, tICS, AiryScan, SIM, STED, SMLM, , Live SR, FRAP, live confocal imaging, Live TIRF and optogenetics)

Quantitative FRET and stoichiometry measurements to map biological activity in cells : fluorescence quantification via an universal QuanTI-FRET protocol

Gene expression, DNA damage, DNA structure and DNA-binding proteins structures, super-resolution (SMLM, RIM, SIM, Airyscan), single particle tracking, photo-manipulation, Correlative Light Electron Microscopy (CLEM)

Atomic force microscopy on living cells or 3D samples is a powerful technique to measure the mechanical properties such as elastic moduli , rigidity, adhesion force, adhesion energy. Combining optical images, AI and AFM, cell population’s mechanome becomes accessible. 3D samples (tissues, organoids) mechanical properties require specific expertise we also provide.

Quantitative measurement of intracellular forces on living cells, but also within tissues (Drosophila, pupae, mouse intestines). Mechanotransduction, micro-nanorheology. High-resolution fluorescence imaging using remote focusing coupled with force measurements.
Compression force measurements