Hight-throughput and high content screening : from sample preparation, automation in sample preparation, data acquisition (plate reader or microscopy), image analysis and statistics analysis (list of hits)
(Key-words: chemical libraries, siRNA libraries, sgRNA CRISPR-Cas9 arrayed libraries covering entire genomes (human and mouse), automatic platform for sample preparation, HCS microscopes with low and high resolution, flow cytometer with images)
Sample specificity: In vitro/purified sample
Single molecule localisation microscopies in different modalities. From sample preparation, labeling, imaging to data analysis.
Forces (nN range) are applied or probed by atomic force microscopy, while samples are correlatively and simultaneously observed by fluorescence microscopy in time-resolved confocal microscopy, epifluorescence or TIRF. Imaging can be coupled to single molecule localisation superresolution microscopy
Measuring biomolecular structure, dynamics and interactions in vitro and in living cells. This includes distances and interactions measurements by single molecule FRET and FRET/FLIM and interactions and dynamics by FCS