T-cell cytotoxic function relies on the cooperation between the highly specific T-cell receptor (TCR) and integrins of co-stimulatory molecule at the immunological synapse. Super resolution 3D STED (Leica SP8, 775 nm) is used to study the oorganisation of these molecules during the activation of immune response. Image cytometer (ImageSteam) allows the quantification of molecules recruited at the immunological synapse in a large number of cells.

To decipher Inflammatory and cardiovascular diseases, multiplex imaging and cytometry pipelines include the entire process from sample preparation, image acquisition to image analysis and quantification. Our workflows are adpated to mouse or human lymphoid organs and blood . They employ techniques such as confocal spectral imaging and cytometry-based analysis (spectral, sorting, and analysis).

Numerous tests to monitor the performance of photonic microscopy systems, including light source power, observation field homogeneity, detector stability, etc. Training at PRIMACEN or at the applicants’ site.

Zeiss CD7, XY, Z-stacks, multi-points, mosaics, time-lapse

With the help of the IBDM Lignt Microscopy Facility we can offer an comprehensive CLEM Pipeline from live fluorescence imaging to EM imaging (in 2D and 3D)

As Volume EM techniques we offer Electron Tomography and Serial Blockface Scanning Electron Microscopy. The targeting of a precise ROI can be achieved by integrating Light Microscopy or X-ray imaging in the pipeline.

Chemical and ultrastructural imaging in electron microscopy for environmental sciences and ecotoxicology: from sample preparation (conventional and cryogenic conditions), to imaging & data processing (Key-words: TEM HR, EDS, STEM-EDS, SEM-EDS, cryoSEM-EDS)

The offer focuses on resolving molecular interactions within living cells by combining advanced quantitative microscopy methods. Techniques such as FLIM, FCS, FRAP, SPT, and super-resolution imaging enable precise analysis of interaction dynamics, diffusion, and nanoscale organization. This integrated approach provides high spatial and temporal resolution for studying biomolecular processes in their native environment.

Our platform offers a dedicated service of bioimage analysis and provide access to powerful workstations, a storage server and a high performance computing cluster with OnDemand. We deploy and maintain for you multiple analysis platforms (Fiji, Napari, QuPath, Imaris, … ), but we can also deploy new tools required for your analysis. With these ressources we can tailor pipelines using state of the art deep-learning solutions to extract quantifiable metrics such as detections, tracks and segmentations from your images.

Depending on the speed and resolution need for your live imaging project , the IGBMC platform propose different imaging systems equipped with an environmental chamber. Wide-field triggered Zeiss AxioObserver system will allow long term analysis of cell motility, wound healing application, Long-term observation of multi-labelled living cells, intracellular dynamics processes analysis (Calcium, ratiometric FRET imaging, vesicles )…High spatial 3D resolution imaging of living cells or tissues is possible on 1 point scanning (Leica TCS SP8X) or on 2 spinning disk microscopes (Yokogawa CSU-X1 or CSU-W1 scanning head) for fast acquisitions with reduced photobleaching. Live-SR modules are available on our 2-spinning disk systems enabling live Super-resolution up to 105 nm at high speed with no specific fluorophores required. 1 TIRF system equipped with a EM-CCD camera allows super resolution imaging along the optical axis (~100 nm) so imaging of membrane processes, such as receptor-ligand interactions, endocytosis, viral infection, or cell adhesion to surfaces.
2 new systems will be installed at the IGBMC platform in Spring 2025, opening up new possibilities for live imaging : 1 Lattice lightsheet will allow dynamic imaging of full sample volumes (cells, spheroids, organoids, zebrafish/c-elegans/drosophilia embryos…) with high temporal and spatial resolution and next to no phototoxicity and bleaching; 1 lattice SIM system will allow cellular, subcellular, and even sub-organelle structures in living specimens imaging in 2D and 3D with high speed, resolution below the diffraction limit (120nm, 60nm with processing) , with no need of specific fluorophores. Highest super resolution live imaging also available at our neighbored FBI Alsace Node “Plateforme Imagerie In Vitro” at INCI with the Leica Steallris STED with TauSTED and Falcon lifetime imaging option.