Phytotronic cabinets and expression of fluorescent fusion proteins/tags (transformation by electroporation)

All equipment necessary for cell culture (PSM, incubators, centrifuges, etc.)

Workflow from sample preparation, MET and cryo-confocal, advanced photonic microscopy
WF/confocal/FLIM, CLEM, image analysis (AMIRA)

Talos F200X G2

HPF, GP2, AFS2 Leica Microsystems

Stellaris 8, WLL, FALCON, Leica Microsystems

X-ray Microtomography of large samples (1mm up to 30cm in diameter)
(Key words : 3D volumetric acquisition; large samples; non-invasive inspection; tomodensitometry)

In vivo visualization of plant tissues cellular development, as well as of their response to climate variations
(Key-words : PICPLANT facility with greenhouses (S2), 3 growth chambers for small and large plants, CONVIRON climatic chamber, histology, stereomicroscope, Spectral-FLIM confocal microscope.

Histology : from sample preparation with the RHEM and PHIV facility (human, animal and plant tissue) to digitisation of virtual slides and image analysis
(Key words : brightfield and multiplex staining, Nanozoomer (brightfield), Axioscan (fluorescence), Pannoramic (brightfield and fluorescence), QuPath and Histometrix with machine/deep learning)