Workflow including sample preparation, 2P microscopy, confocal microscopy, light sheet microscopy, ophthalmic imaging/OCT/ERG and image analysis.

Workflow including sample preparation, light sheet microscopy, confocal microscopy, intravital microscopy, and image analysis.

Inscoper module for control, Nikon FNE stand, Yokogawa CSU W1 head, two cameras

Ultima 2Plus Bruker, Chameleon vision II
Second laser 1000-1300 nm.

Mono and bi-photon longitudinal imaging with flexible windows and/or stabilizing chambers for imaging peripheral organs (e.g. heart, lung, liver, gut, pancreas) and human organoids/spheroids/tumoroids implanted in mouse or zebrafish models. This provides information about how they get connected to the blood vasculature.

Longitudinal imaging with one/two colour miniscope with high speed acquisition. Pre-implantation of mouse models with GRIN lenses (objectives from 4 to 7.6mm long and 0.6 to 1.4mm diameter) in brain region expressing specific fluorescent reporters. Mice are imaged for days to several months and fluorescent signals can be correlated with cognitive, behaviour and physiological outcomes.

Intravital mono or bi-photon imaging with either short or long (up to 2 cm) working-distance objectives and coupled with cellular manipulation (mouse, fish and insect models). This can be applied to high resolution cellular imaging of molecule extravasation (e.g. nanobodies, drugs, hormones), capillary blood flow, cellular migration and signaling (e.g. calcium, ligand sensors), and molecule distribution into the extracellular space.

Acquisition of images using confocal/multiphoton macroscopes/microscopes in dorsal chambers or on surgically exposed organs (without chamber).

Multi-modality dorsal chamber (photonics, MRI, ultrasound) enabling long-term longitudinal monitoring (several weeks). Performance (in collaboration or team training) of surgical transplants and high-resolution image acquisition using macroscopes/confocal/multiphoton microscopes.