The In Vitro Imaging platform has recognized expertise in confocal, super-resolution, transmission, and scanning electron microscopy, as well as in a wide range of sample preparation techniques, including cryofixation and cryosubstitution. These techniques are applied to brain tissue, primary and organotypic cell cultures, as well as membrane sheets, enabling the detailed study of functional and pathological processes in neuronal and neuroendocrine cells, with a focus on intracellular signalling pathways.