the imaging system Kratoscope acquires an image of the organ in the block of tissue each time a slice is cut by a microtome (FFPE blocks) or a cryostat (frozen tissue), in a fully automated process. By getting a stack of slices at increasing depth, it is then possible to reconstruct a 3D view of the organ.
In addition to a possible fluorescent signal detection, the contrast of the images comes from the difference in autofluorescence of different structures of the organ and highlights anatomical its anatomy. It can provide information not visible on micro CT images, when small structures lack radio contrast.