High content microscopy with Nikon’s confocal LIPSI system combines automated image acquisition with integrated analysis, making it particularly powerful for high-content screening and live-cell studies. By reducing manual steps such as focusing, exposure adjustment, and data processing, it ensures reproducibility and standardization across experiments. This system is equipped with a robotic arm for scanning up to 20 multi-well plates or 80 slides on both fixed and live samples. A colour camera is also available for combining fluorescence acquisition with histological staining.