We propose a workflow to study fast biological phenomena in living in immune tissues or cells. We have a mammalian cell culture room to manipulate and preserve the cells under optimal conditions before acquisition. Our microscopy system is specifically designed to investigate cell motility and vesicular traffic in 4D (3D plus time) using fast confocal imaging. For this purpose, we use an inverted microscope (a Zeiss Axio Observer Z1) equipped with an incubation chamber (CO2, humidity and temperature) a spinning disk fast confocal module (CSU X1 Yokogawa confocal head) and also an improved resolution module (Gataca LiveSR). Long time acquisition are possible thanks to a focus stabilization (definite focus). Additionally, diffusion or transport of fluorescent molecules can be studied with FRAP (Fluorescence Recovery After Photobleaching) and other F technics thank to the Ilas module (Gataca systems).