Sample preparation is essential to observe biological samples in electron microscopy. Imagerie-Gif facility propose all the equipment to cover a large diversity of application: chemical fixation, high pressure freezing, resin embedding, ultramicrotomy and cryo-ultramicrotomy. They also propose elaboration of immunolabeling on resin section or cryosections. The observation of sections are made by TEM for high resolution imaging, or by SEM for large field and 3D acquisition.