High speed, low photobleaching with sub cellular resolution and high detection sensitivity for cleared whole organs, brains or small embryos is offered by the Zeiss light Sheet LS7 -Multiphoton confocal system also available depending on specific applications (including Second Harmonic Generation), large field of view spinning disk (Leica microscope equipped with Yokogawa CSU-WI scanning head) also available and can be used for living sample. Both multiphoton confocal and spinning disk are equipped with 20x, 0.95 NA Water IMM objective providing 2.2 mm free working distance allowing in depth imaging with an excellent 3D resolution in tissue, small animal models, organs, organoids…
Following acquisition, solution or assistance for 3D image analysis, segmentation and quantification can be provided using dedicated software (Imaris, Cellpose, Napari, specific Python scripts…) For this purpose, the facility is equipped with several GPU workstations for efficient processing of big data, available to users.