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2D or 3D electron microscopy morphometric analysis of the central nervous system
2D or 3D electron microscopy morphometric analysis of the central nervous system with Deep FIB-SEM and IA-based image analysis
3D cellular or tissue imaging by electron microscopy
3D cellular or tissue imaging by electron microscopy (SerialBlock Face SEM and thick sections in electron tomography)
3D imaging of spheroids and organoids
The Evident Ixplore workstation equipped with the Yokogawa CSU-W1 scan head, Hamamatsu orca-fusion camera and the UPLSAPO Si/1.05 30x objective is a powerful tool for acquiring clarified spheroid and organoid…
3D life imaging of organoids & thick samples
High-Throughput Light Microscopy for Organoids: Preparation, Imaging (spinning disk; CLSM; 2-photon) and Analysis
3D Spatial transcriptomics (EQUIPEX Spatial-Cell-ID)
The Spatial-Cell-ID facility aims to enhance the spatial resolution of MERFISH for pinpointing transcripts of specific genes in situ
Advice and production of image competitions and photo exhibitions
Advice and production of image competitions and photo exhibitions (Keywords : rules for an image competition, totem for outdoor display, dibond printing)
AFM, multi-scale and multimodal force measurement
Atomic force microscopy on living cells or 3D samples is a powerful technique to measure the mechanical properties such as elastic moduli , rigidity, adhesion force, adhesion energy. Combining optical…
Ageing/geroscience: multimodal, multispectral, multiparametric, multiscale, multispecies analysis
To tackle complex biological questions in aging and geroscience, we design and implement advanced multimodal, multiparametric, and multiscale pipelines in imaging, flow cytometry, and image flow cytometry. Our workflows span…
AI-assisted analysis of muscle biopsies
Comprehensive quantitative analysis pipeline for muscle biopsies, integrating advanced imaging and artificial intelligence tools to extract precise morphological and functional parameters.
Analysis of immune signalling at the cellular level (Image cytometry – Analysis of the immune synapse using 3D STED)
T-cell cytotoxic function relies on the cooperation between the highly specific T-cell receptor (TCR) and integrins of co-stimulatory molecule at the immunological synapse. Super resolution 3D STED (Leica SP8, 775…
ASB3 microscopy (lungs infected with level 3 pathogens)
Atomic-scale 3D structural analysis by cryo-electron microscopy on cells and tissues (cryo-lamella by cryo-FIB-SEM, cryo-TEM, cryo-SEM)
We combine latest developments in cryo sample preparation and imaging by electron microscopies (cryo-FIB/SEM and cryo-TEM) to observe biomolecules of interest (ribosomes, carboxysomes, viruses, nuclear pore complexes…) within their cellular…
Automated multi-position, phase contrast and fluorescence microscopy
Zeiss CD7, XY, Z-stacks, multi-points, mosaics, time-lapse
Bioimage analysis support: Development of tailored analysis pipelines for your project.
Our platform offers a dedicated service of bioimage analysis and provide access to powerful workstations, a storage server and a high performance computing cluster with OnDemand. We deploy and maintain…
Biology applied to oncology: sample preparation, spatial analysis using multiplex imaging, single-cell flow cytometry
Inflammatory and cardiovascular diseases are complex, multifactorial conditions that affect multiple organs and cellular components. To tackle these intricate biological challenges, we employ and advance multi-modal and multi-scale imaging and…
Cell-biology consulting for physicist, chemist or bioimage analyst
Cell-biology expertise: providing cells(epithelial, glial, neuronal) and organoïds, labelling (transfections or probe-Test), and imaging for physicist, chemist that would like to test a prototype. Including : Guidance on super-resolution modalities…
Cellular imaging for neurodegenerative diseases studies
Cellular imaging for neurodegenerative diseases studies : nervous system, neurons in culture, cell biology, cytoskeleton
Characterisation of the immune and inflammatory response of cells to tissue using flow cytometry and multiplexed confocal imaging
To decipher Inflammatory and cardiovascular diseases, multiplex imaging and cytometry pipelines include the entire process from sample preparation, image acquisition to image analysis and quantification. Our workflows are adpated to…
Chemical and ultrastructural imaging in electron microscopy for environmental sciences and ecotoxicology
Chemical and ultrastructural imaging in electron microscopy for environmental sciences and ecotoxicology: from sample preparation (conventional and cryogenic conditions), to imaging & data processing (Key-words: TEM HR, EDS, STEM-EDS, SEM-EDS,…
Chromatin structure and dynamic
Gene expression, DNA damage, DNA structure and DNA-binding proteins structures, super-resolution (SMLM, RIM, SIM, Airyscan), single particle tracking, photo-manipulation, Correlative Light Electron Microscopy (CLEM)
CLEM RT (Room Temperature)
With the help of the IBDM Lignt Microscopy Facility we can offer an comprehensive CLEM Pipeline from live fluorescence imaging to EM imaging (in 2D and 3D)
Cochin Image Database
Online database for saving, archiving, analyzing, and processing images. Features include 2D and 3D segmentation using deep learning, denoising, and 3D deconvolution
Confocal microscopy
2 Leica SP8 confocal microscopes, equiped with HyD detectors and classical PMT detectors. The facility has expertise in practical training, counseling users in IF protocols, sample mounting and preparation.
Controlled pressure SEM imaging on hydrated samples, in L2 biosafety laboratory
Expertise in preparing and observing biological samples in their native form—hydrated or prepared by fixation and dehydration via critical point bypass—using the Hitachi SU5000 SEM. Analysis of microorganism morphologies.
Core Facility Management Software
Core Facility Management Software (home-made development) (Keywords : user management, instrument management, booking website, user autonomy management, dysfunction management, usage reports at different levels, invoicing)
Correlative light and electron microscopy
Connecting different resolutions for correlative confocal/SEM and confocal/TEM on adherent cells
Correlative light/electron microscopies imaging for plant sciences: from sample preparation, to imaging & data processing
Correlative light/electron microscopies imaging for plant sciences: from sample preparation, to imaging & data processing (Key-words: cryo-preparation for resin embedding, electron tomography, correlation, image analysis)
Correlative light/electron microscopy (CLEM)
Correlative light/electron microscopy (CLEM)
Cryo-CLEM
CryoCLEM microscopy combines cryo-electron microscopy (CryoEM) with cryo-light microscopy, enabling the correlation of molecular localization with high-resolution structural details in frozen samples. This approach preserves the native state of biological…
Cryo-confocal-FLIM microscopy
Stellaris 8, WLL, FALCON, Leica Microsystems
Cryo-EM
We use Cryo-electron microscopy to capture high-resolution images of frozen molecules, liposomes and other thin specimens, preserving their natural structure.
Cryo-ET of in vitro reconstituted samples
Cryo-ET of in vitro reconstituted samples, extracellular vesicles, and organelles OR electron tomography at room temperature of thick sample (300 nm)
Cryo-nano CLEM: Super-resolution-based correlative light/electron microscopies imaging in cryogenic conditions for neurosciences. From sample preparation, to imaging & data processing
Cryo-nano CLEM: Super-resolution-based correlative light/electron microscopies imaging in cryogenic conditions for neurosciences. From sample preparation, to imaging & data processing (Key-words: STORM, PALM, PAINT, DNA-PAINT, SRRF, PALM-Tracer, cryo-TEM, tomography, data…
CUBIC transparency and lightsheet imaging of tissues and embryos
Expertise in preparing biological samples using CUBIC transparisation, LS7 Zeiss light sheet microscopy imaging, and working with volumetric images (size reduction, segmentation).
Culture room for microalgae and fluorescent protein expression in microalgae, phytotron for plants
Phytotronic cabinets and expression of fluorescent fusion proteins/tags (transformation by electroporation)
Development of complex acquisitions (smart Microscopy)
The automation of fluorescence microscopy represents a challenge for the study of rare or transient events in biology and medicine. It relies on intelligent devices that integrate and interpret observed…
Development of image analysis solutions using artificial intelligence
Jupyter notebooks, visualisation and deep learning libraries (StarDist, CellPose, etc.)
Development, observation and identification of specific markers on 3D models and tissue
support from sample preparation to image analysis – including visualisation of organoids/spheroids in 3D imaging. Pool of systems and expertise for these studies (transparization, staining, light sheets, etc.).
Direct link to pet shops (conventional/EOPS) for small and large animals (Rouen – Caen) and aquariums for marine invertebrates (Rouen)
Rats, mice, non-human primates, mussels, shrimps, copepods
Dynamic imaging on multicellular systems (FRAP, FLIM)
Dynamic imaging on multicellular systems (FRAP, FLIM)
Electron microscopy: from sample preparation to image analysis
The platform offers advanced cryofixation protocols, using a high-pressure freezer (Wohlwend Compact 03) and a cryosubstitution unit (Leica AFS). Sections are prepared by ultramicrotomy and observations are made by TEM…
Expansion Microscopy
ExM cell & tissue : ProExM, TREx, ExSTED ExPath
Explanted organs (biphotonic imaging)
Maintain of the mouse living organs under 2P microscope Bruker 2P+, thanks to perfusion and oxygenated medium
Fast & Sensitive live imaging at nano-scale level
Low signal fast live imaging at nano-scale level (Airy / Fast Airyscan , Live-3D-STED, Live-3D-SLMN)
Fast wide-field FLIM imaging (TIRF/Hilo/epi), measurement of local tension or protein-protein interactions
Resolved fluorescence acquisition combining a supercontinuum pulsed laser and wide-field detection (time-gated), with the possibility of TIRF/hilo-type optical sectioning. This device allows the observation of biosensors and/or the monitoring of…
FCS with variable waist on cells
We use homemade microscope dedicated with high sensitivity and Z scan to choose the right focus on cells compartments
FLIM microscopy to study protein-protein interactions and specific probes in plant tissues (multisite)
FLIM-STED nanoscopy
Stellaris 8, WLL, FALCON, 592 and 775 nm for depletion, Leica Microsystems
From organ to subcellular analysis of immune tissues
We offer workflows that enables tissular to subcellular analysis with a specialization in in immune tissues. Our team provides guidance and support at key stages throughout the process. We offer…
Functional and pathological changes in neuronal and neuroendocrine cells: characterization of intracellular signalling networks
The In Vitro Imaging platform has recognized expertise in confocal, super-resolution, transmission, and scanning electron microscopy, as well as in a wide range of sample preparation techniques, including cryofixation and…
Functional microscopy (FLIM-FCS, FRET, FRAP, laser ablation)
Functional microscopy at the molecular, cellular, and tissue levels (FLIM-FCS, FRET, FRAP, laser ablation)
GR dorsal chamber: implantation surgery and confocal/multiphoton imaging
Multi-modality dorsal chamber (photonics, MRI, ultrasound) enabling long-term longitudinal monitoring (several weeks). Performance (in collaboration or team training) of surgical transplants and high-resolution image acquisition using macroscopes/confocal/multiphoton microscopes.
High resolution imaging of cell signals and blood flow with miniscopes (<2.5g weight) in freely-behaving mouse models
Longitudinal imaging with one/two colour miniscope with high speed acquisition. Pre-implantation of mouse models with GRIN lenses (objectives from 4 to 7.6mm long and 0.6 to 1.4mm diameter) in brain…
High resolution volumic microscopy
The facility is equiped with a FIB-SEM (Focused Ion Beam-Scanning Electron Microscopy), that allows volumic acquisition of resin embedded samples at nanometric resolution (up to 5nm in 3D). This technology…
High temporal resolution, particularly for ionic flows
Measuring Ca²⁺ or pH in microscopy is important because both are fundamental regulators of cellular activity. Calcium controls processes such as signaling, contraction, and secretion, while pH influences enzyme activity,…
High throughput and high content screening
Hight-throughput and high content screening : from sample preparation, automation in sample preparation, data acquisition (plate reader or microscopy), image analysis and statistics analysis (list of hits) (Key-words: chemical libraries,…
High throughput slide production, staining and digitisation services
Our 2 platforms offer routine services in histology, tissue processing, thin sections, staining, immunohistochemistry and digitisation of microscopy slides (Hamamtsu S360 & S60 scanners).
High-pressure, automatic immersion freezing system, cryo-substitution automaton
HPF, GP2, AFS2 Leica Microsystems
High-resolution imaging + EXM
Confocal microscopy or/and spinning disk microscopy combined with sample expansion technique. Boyden and al.
High-resolution imaging coupled with expansion microscopy
Expertise in preparation of sample in expansion microscopy coupled with microscopy high resolution and lightsheet
High-throughput confocal imaging (high content screening) for bacterial biofilm analysis, in L2 biosafety laboratory
Expertise in Leica SP8 multi-position HCS confocal imaging in 98-well plates for spatial analysis of microbiological biofilms.
Histology : from sample preparation with the RHEM and PHIV facility (human, animal and plant tissue)
Histology : from sample preparation with the RHEM and PHIV facility (human, animal and plant tissue) to digitisation of virtual slides and image analysis (Key words : brightfield and multiplex…
Holotomography (label-free, long-term imaging)
Holotomography merges holography and tomography to solve the challenge of recovering light phase shifts to generate image contrast in Quantitative Phase Imaging. HT illuminates a cell with very low power…
Image analysis (collaborative projects) – F-BIAS
Creation of customised image analysis pipelines tailored to meet the needs of an imaging project.
Image analysis (collaborative projects) – Pasteur Institute
Creation of customised image analysis pipelines tailored to meet the needs of an imaging project.
Image analysis (consultations) – F-BIAS
Consultations on image analysis, to address a specific issue in a project.
Image analysis (consultations) – Pasteur Institute
Consultations on image analysis, to address a specific issue in a project.
Image analysis (training/contracting)
Python, ImageJ, Fiji, QuPath,plugin, macro, notebook, deep-learning and many others
Imaging for cardiovascular sciences
Workflow including sample preparation, light sheet microscopy, confocal microscopy, intravital microscopy, and image analysis.
Imaging for neurovascular sciences
Workflow including sample preparation, 2P microscopy, confocal microscopy, light sheet microscopy, ophthalmic imaging/OCT/ERG and image analysis.
Imaging, quantifiying and controlling cell metabolism
Imaging, quantifiying and controlling cell metabolism (FLIM, TIRF-FastFLIM, ratipmetric-SD, optogenetis…)
In vivo imaging for neurosciences: labelled & label-free sample imaging
In vivo imaging for neurosciences: labelled & label-free sample imaging (Key-words: Two-photon imaging, SHG)
In vivo visualization of plant tissues cellular development, as well as of their response to climate variations
In vivo visualization of plant tissues cellular development, as well as of their response to climate variations (Key-words : PICPLANT facility with greenhouses (S2), 3 growth chambers for small and…
Intravital 1P/2P imaging and cellular manipulation
Intravital mono or bi-photon imaging with either short or long (up to 2 cm) working-distance objectives and coupled with cellular manipulation (mouse, fish and insect models). This can be applied…
Intravital imaging (dorsal chamber, exposed organs)
Acquisition of images using confocal/multiphoton macroscopes/microscopes in dorsal chambers or on surgically exposed organs (without chamber).
Intravital imaging (murin or tissue of human origin)
Intravital imaging (murin or tissue of human origin) BSL-2 multi-photon spectral FRET-FLIM, SHG THG (collagen I & III, myosin)
Intravital imaging: Non-infected or transparentised organs and cancerous tumours (in NSB1)
Bruker 2P+, Discovery laser (680 to 1340 nm), mouse anesthesy and stabulation. Cleared mouse organs (gut, lymph nodes..) and 2P microscopy
Intravital multiphoton imaging
Kratoscope (system based on autofluorescence or labelling installed on microtome or cryostat for 3D imaging and recovery of sections)
the imaging system Kratoscope acquires an image of the organ in the block of tissue each time a slice is cut by a microtome (FFPE blocks) or a cryostat (frozen…
Label free molecular imaging: SRS / CARS
We use different imaging modalities to probe chemical fingerprinting in pharmalogical drug studies or cancer diagnosis. Many types of samples can be imaged such as liquids, powders, cells, tissues, organisms
Label-free imaging: CARS confocal Raman microscopy, SRS coupled with SHG
Large scale light microscopy for neurosciences: from sample preparation, to imaging & data processing
Large scale light microscopy for neurosciences: from sample preparation, to imaging & data processing (Key-words: transparisation, expansion microscopy, confocal microscopy, two-photon imaging, light-sheet microscopy, lattice-light sheet microscopy, Multimodal Optical Scope…
Laser nano dissection in tissues
Light microscopies for plant sciences: from sample preparation, to imaging & data processing
Light microscopies for plant sciences: from sample preparation, to imaging & data processing (Key-words: immunolabelling, whole mount, slices, immuno gold, FISH, photomanipulation, live/dynamic imaging)
Light sheet microscopy
Multidirectional illumination for transparent living samples (Alpha3 facility edition Phase View) and XXL chamber for transparent samples (Ultramicroscope Blaze, Milteniy BioTech)
Light-sheet and biphoton imaging
Full pipeline support on light sheet and biphoton imaging (from sample prep and tissue clearing to imaging)
Light-sheet imaging
Strong expertise in sample preparation, transparisation, handling and imaging for Lightsheet microscopy
Light-sheet imaging in Biology Safety Level 2
Light-sheet imaging of fixed and live samples. We can afford (cells, IPS, organoïds, hydra, fish, human tissues) because the microscope is installed in Biology Safety Level 2
Lightsheet: with clarification or not
We work on mouse models (embryos at different stages, mouse organs, tumor samples) with solvent based clearing methods (iDisco modified, home made protocols depending on the samples).
LIPSI (robotised confocal HCS and HTS, with Smart microscopy capability)
High content microscopy with Nikon’s confocal LIPSI system combines automated image acquisition with integrated analysis, making it particularly powerful for high-content screening and live-cell studies. By reducing manual steps such…
Liquid Liquid Phase Separation (LLPS) in vitro and in cellulo caracterization
The study of membrane-less compartmentalisation in cells is a growing field. At Imagerie-Gif, we have developed methods to identify these phenomena and quantify their dynamics using light microscopy techniques (DIC/FRAP/tracking,…
Live imaging and molecular dynamics for cell, organoids, tissue and embryo
Live Imaging and molecular dynamics for cell, organoids, tissue and embryo : from sample preparation with access to a L2 cell culture room, to microscopy and data analysis (Key words…
Live imaging in confocal of immune tissues or cells
We propose a workflow to study fast biological phenomena in living in immune tissues or cells. We have a mammalian cell culture room to manipulate and preserve the cells under…
Localisation of unique molecules in complex/deep environments (ModLoc) (axial precision below 7 nm)
STORM/DNA-PAINT acquisition for in-depth and/or complex environment observation, with uniform precision thanks to axial position encoding in structured illumination.
Longitudinal intravital imaging of human organoids/spheroids implanted in small animal models
Mono and bi-photon longitudinal imaging with flexible windows and/or stabilizing chambers for imaging peripheral organs (e.g. heart, lung, liver, gut, pancreas) and human organoids/spheroids/tumoroids implanted in mouse or zebrafish models.…
Macro-Image J development and scripts
Groovy for Q-Path
Mechanics of biological samples with correlative AFM / fluorescence.
Forces (nN range) are applied or probed by atomic force microscopy, while samples are correlatively and simultaneously observed by fluorescence microscopy in time-resolved confocal microscopy, epifluorescence or TIRF. Imaging can…
Meso- and macro-imaging of marine invertebrates
Workflow including sample preparation, light sheet imaging, and fluorescence macroscopy
Metrology of photonic imaging systems
Numerous tests to monitor the performance of photonic microscopy systems, including light source power, observation field homogeneity, detector stability, etc. Training at PRIMACEN or at the applicants’ site.
Microalgae imaging
Workflow from sample preparation, MET and cryo-confocal, advanced photonic microscopy WF/confocal/FLIM, CLEM, image analysis (AMIRA)
Molecular analysis of plant tissue: protein-protein interactions and chemical mapping (FRET-FLIM, Raman), transmission electron microscopy
Molecular dynamics and interaction (F-techniques)
molecular diffusion by FRAP, FCS and SPT; macromolecular interactions by FRET-FLIM, fluorescence anisotropy and FCS-2colours; biochemical activities by FRET biosensors
Morphological analysis of neural tissues by TEM
Expertise in preparation and morphological analysis using TEM (brain and spinal cord)
Most photonic microscopy in the L2 zone. L2 for animal/plant cell culture.
All equipment necessary for cell culture (PSM, incubators, centrifuges, etc.)
Multi-colour TIRF microscopy
IX83 microscope equipped with two PENTA SET TIRF, detector (sCMOS ORCA Fusion BT), TIRF module (445nm, 488nm, 514nm, 561nm, 640nm), cellVivo module, cellSens software (Evident)
Multi-modal and multi-scale analysis of inflammatory, cardiovascular and dyslipidaemic diseases
Inflammatory and cardiovascular diseases are complex, multifactorial conditions that affect multiple organs and cellular components. To tackle these intricate biological challenges, we employ and advance multi-modal and multi-scale imaging and…
Multi-modal Imaging (4D, CARS, FCS, FLIM….) of Risk 2/3 biological samples
On demand from basic training for image acquisition to full pipeline support in multi modal imaging
Multicontrast multiphoton imaging – 2P / SHG / THG / 3P / polarization / FLIM
Strong local expertise in multi-contrast multiphoton tissue imaging based on 2PEF, 3PEF, SHG, 3PEF, polarization and ZP-FLIM contrasts. Acces to lab-built and application-oriented systems for pilot studies after a project…
Multimodal correlative microscopy, including CLEM
Multimodal microscopy, by combining fluorescence, harmonic and electron microscopy signals, is therefore of real interest for taking advantage of complementary information and increasing the sensitivity of pathology detection to qualify…
Multimodal plant imaging: integrating multiple techniques for comprehensive plant analysis
The Imaging Facility at IBMP-CNRS provides researchers with access to state-of-the-art microscopy techniques, including fluorescence, confocal, and 3D scanning electron microscopy. The platform supports a variety of sample preparation workflows,…
Multiparametric imaging flow cytometry
High-resolution multiparametric quantitative analysis using ImageStream® MkII technology, combining imaging and flow cytometry to precisely characterise cells.
Multiplex imaging
makes it possible to observe a large number of biomarkers simultaneously and to identify the diversity of cell populations and their interactions within a sample. Immunofluorescence imaging offers high spatial…
Multiscale imaging of complex 3D objects: from embryonic to subcellular imaging
Multiscale imaging of complex 3D objects: from embryonic to subcellular imaging (adaptive confocal and two-photon microscopy, light-sheet imaging of cleared tissues; NIR detection of luminescent probes, IA-based image analysis)
Multiscale live imaging: from cell motility to intracellular processes dynamics analysis with super-resolution
Depending on the speed and resolution need for your live imaging project , the IGBMC platform propose different imaging systems equipped with an environmental chamber. Wide-field triggered Zeiss AxioObserver system…
Multiscale measurements of molecular dynamics in cell and tissue
Multiscale measurements of molecular dynamics in cell and tissue (SPT, FCS, tICS, AiryScan, SIM, STED, SMLM, , Live SR, FRAP, live confocal imaging, Live TIRF and optogenetics)
Multisite spatial transcriptomics
Exploration of gene expression in its tissue context, using a spatial transcriptomics approach deployed across multiple sites, enabling detailed and comparative mapping of transcriptomic profiles.
Muscle analysis
PIC-STRA offers solid experience in muscle sample preparation (cutting, fixing, marking). Thanks to the multiple acquisition systems available (wide field, confocal, super-resolution, slide scanner + deconvolution), our platform provides tools…
Nanoscopie FLIM-STED
Visualization of sub-diffraction-limit cellular structures (
Nanoscopy (STED 3D, SMLM, DNA-PAINT, 3D SIM)
3D SIM on microorganisms
Negatif staining analysis
Single-particle analyses performed using TEM after negative staining
nm-characterization of multitypes materials by Cryo-TEM/SEM, and electron tomography
nm-characterization of multitypes materials like liposomes, emulsion, hydrogel and porous materials by Cryo-TEM/SEM, and electron tomography,
On-demand workflow design for image processing and analysis
On-demand workflow design for image processing and analysis
On-demand workflow design for image processing and analysis
On-demand workflow design for image processing and analysis (Key-words: Python, Java, ImageJ, Fiji, plugin, macro, notebook, deep-learning)
Organelles and cell sorting (L1 and L2 environment) in Multisite
Identifying your cell or organelle populations according to morphology and/or fluorescence allows you to sort and purify them in tubes (from Eppendorf 1.5 ml to Falcon 50 ml) or plates…
Organoids: from preparation to analysis
Organoids (lung, endometriosis, digestive tract, adipose tissue, bone marrow, skin, bladder, fallopian tube): integrative analysis (sample preparation, acquisition – light sheet, confocal, two-photon, high-content screening, multiparametric analysis, 3D imaging, analyses,…
Photomanipulation of biological objects
Photomanipulation of biological objects (photoablation on spinning disks and on confocal equipped with 2photons, optogenetics)
Photon Up-Conversion Characterization and Applications for Bioimaging
The offer provides state-of-the-art instruments optimized for the detailed characterization of upconversion nanoparticles (UCNPs). These systems enable the study of their photophysics and stability, as well as their application in…
Plant and insects genome size analysis by cytometry with automated detection
It is simple to measure genome size (pg DNA) of plants or insects with cytometry by isolating nuclei mechanically in adequate buffer.
Plant and insects genome size analysis by cytometry with automated detection
Polarization-resolved fast confocal microscopy (molecular organization and orientation)
We use multiple imaging systems to probe the polarization of fluorescent molecules. This gives an access to their molecular organization and orientation within cells and organisms.
Protein and molecular dynamics imaging and analysis by FRAP, FRET-FLIM in cells
In order to characterise dynamic phenomena at the molecular level, we have developed expertise in evaluating their diffusion (FRAP) and highlighting interactions (FRAP/FRET-FLIM).
Protein and molecular dynamics imaging and analysis by FRET-FLIM in cells and biosensors FLIM imaging
Fast detection of molecular interactions and microenvironmental changes is enabled by the LEICA Stellaris 8 confocal microscope, integrated with the FALCON (FAst Lifetime CONtrast) module for Fluorescence Lifetime Imaging (FLIM).…
Quantification of Biomolecular Interactions and Molecular Dynamics
The offer focuses on resolving molecular interactions within living cells by combining advanced quantitative microscopy methods. Techniques such as FLIM, FCS, FRAP, SPT, and super-resolution imaging enable precise analysis of…
Quantification of density and structure of biofunctional surfaces
Quantification of density and structure of biofunctional surfaces (FRAP and reflection interference contrast microscopy-RICM) and nanometric dynamic cell-substrate distance mapping
Quantifying Physical parameters from animal/plant cell to tissue
Quantifying Physical parameters (rigidity, tension force production, turgor pressure…) from animal/plant cell to tissue (TFM, Brillouin imaging, photoablation, Triboindenteur, AFM, AFM Hybrid Stage)
Quantitative FRET and stoichiometry measurements to map biological activity in cells
Quantitative FRET and stoichiometry measurements to map biological activity in cells : fluorescence quantification via an universal QuanTI-FRET protocol
Quantitative imaging of bio or mecano-sensors using ratiometry, FLIM or cytometry approaches
Light microscopy enables biosensors to be used to characterize the cellular environment (pH, ROS, calcium, etc.) in order to highlight differences between populations or identify heterogeneities within tissues or cells…
Quantitative phase imaging
We use QPI to quantify the dry mass, intra-cellular transport, cell growth, cell migration
Raman spectroscopy imaging, CARS, Stimulated Raman Scattering
Imaging by Raman spectroscopy (RAMAN DXR2xi imager, ThermoFisher Scientific): each molecule has a unique spectral signature, determined by the Raman photons emitted after interaction with light. These photons are collected…
Random or structured 3D super-resolution imaging or AO: medium-throughput RIM system for small objects (cells, bacteria, yeasts) or tissue and organoids
RIM (Random Illumination Microscopy): In depth Super resolution with wide-field excitation
RIM, based on natural laser speckle illumination, is a method of choice for super-resolved imaging of living cells in depth. The idea is to combine optically resolved laser speckle illumination…
Root growth monitoring by microfluidics
RT and cryo transmission electron microscopy
Talos F200X G2
Scanning electron microscopy in cryogenic conditions for plant imaging, animals imaging and soft materials
Scanning electron microscopy in cryogenic conditions for plant imaging and soft materials: from sample preparation, to imaging & data processing (Key-words: HPF, plunge freezing, cryofracture, cryoSEM, cryoSEM EDS)
Second harmonic generation and STED correlative microscopy
Correlation microscopy between super-resolution acquisition and collagen harmonic signalling
Single molecule imaging for neurosciences: from sample preparation, to low and high throughput imaging & data processing
Single molecule imaging for neurosciences: from sample preparation, to low and high throughput imaging & data processing (Key-words: SPT-PALM, STORM, PALM, PAINT, DNA-PAINT, High throughput imaging in super-resolution, PALM-Tracer, data…
Single molecule microscopy
Full pipeline support on single molecule microscopy equipment (from sample to data analysis)
Single Molecule mRNA Imaging
Single Molecule mRNA Imaging : smFISH in 96 plates and low-troughput multiplexed smFISH in collaboration with Edouard Bertrand’ lab, from probe design and handling to data pre-processing and training for…
Single molecule RNA FISH
Full pipeline support for single molecule RNA FISH
Small Instrument Prototyping for Imaging: Open Hardware and Frugal Science Approaches
The Imaging Facility at IBMP-CNRS can help researchers design, adapt, build or automate original pieces of laboratory equipment in the field of biological imaging.
Smart microscopy with deep learning on medium-throughput cellular imaging
Spatial analysis of gene expression in plants (laser microdissection and in situ hybridisation)
Spatial super resolution (Airy Scan, NSIM, RIM, NSpark, STORM Abbelight)
spatial super resolution by ISM (Image Scanning Microscopy) with AiryScan and NSpark in confocal (Zeiss and Nikon); by structured illumination with NSIM and RIM (Nikon and prototype); by SMLM with…
Spectral confocal microscopy
We use Zeiss LSM 980 NIR with large possibilities of laser excitation. We propose panel design to acquiistion and imag analysis with QuPath
Spinning disk microscopy
We are equiped with one Nikon +Yokogawa W1 spinning disk, 6 wavelenghts. We propose technics like TIRF, FRET, FRAP and LiveSR (Gataca solution). The facility has strong expertise in dynamic…
Static and live imaging in confocal to investigate cell behavior / motility on surfaces
Depending on your imaging project’s requirements for speed and resolution, the IS2M platform offers a comprehensive range of advanced imaging systems equipped with environmental chambers. This includes: • A spinning…
STED correlative imaging with molecular dynamics
STED correlative imaging with molecular dynamic and interaction (FCS-FLIM-FRAP-FRET) on thin, thick samples
STED microscopy coupled with an atomic force microscope
Super-resolution acquisition coupled to high-end atomic force microscopy
Straight spinning disk microscope for intravital imaging
Inscoper module for control, Nikon FNE stand, Yokogawa CSU W1 head, two cameras
Structure and dynamics of biomolecules using single molecule FRET, time-resolved & correlation methods
Measuring biomolecular structure, dynamics and interactions in vitro and in living cells. This includes distances and interactions measurements by single molecule FRET and FRET/FLIM and interactions and dynamics by FCS
Study of forces
Quantitative measurement of intracellular forces on living cells, but also within tissues (Drosophila, pupae, mouse intestines). Mechanotransduction, micro-nanorheology. High-resolution fluorescence imaging using remote focusing coupled with force measurements. Compression force…
Studying cellular processes, probing the functionality of specific proteins and organelles using photomanipulation techniques within living samples
Several modules or lasers are available for specific photomanipulation experiments at the sub-cellular level: high energy 355 nm pulsed lasers allow uncageaging, DNA damage induction, or precise photoablation (available on…
Super-resolution imaging (STED)
STEDYCON Microscope Confocal and STED techniques all in one system. 775 STED laser + 405-488-560-640 lasers
Super-resolution imaging in plants (dSTORM, STED)
Super-resolution in different modalities (single particle tracking, dSTORM, DNA-PAINT and PALM)
Single molecule localisation microscopies in different modalities. From sample preparation, labeling, imaging to data analysis.
Super-resolution microscopy
Airyscan, SIM, STORM modes
Super-resolution-based imaging for cell, tissue and embryo
Super-resolution-based imaging for cell, tissue and embryo : from sample preparation, to imaging & data processing (Key-words: Airyscan, STED, STORM, PALM, expansion microscopy alone or combined with other super-resolution technics,…
Super-resolution-based imaging for neurosciences: from sample preparation, to imaging & data processing
Super-resolution-based imaging for neurosciences: from sample preparation, to imaging & data processing (Key-words: STED, STORM, PALM, PAINT, DNA-PAINT, SRRF, PALM-Tracer, data analysis)
Super-resulution microscopy
:STED-FLIM BSL-2 : Live / 3D / multi-labeling
Support for the design and implementation of a cytometry experiment
Support for the design and implementation of a cytometry experiment (Keywords : panel design, sample preparation, data acquisition, data sorting and analysis)
Surface morphology and ultrastructural analysis
Full workflow support on surface morphology and ultrastructural analysis in SEM and TEM
Synthesis of probes for chemobiology by bioconjugation of fluorophores with biologically active molecules
Complete workflow from organic chemistry to fluorescence analysis (time-gated, TCSPC and MSC)
Tissue clearing
:Service, Whole organ, Thick tissue cutting 500µm, Immuno-labeling
Training portfolio
Training portfolio (BioCampus workshops recognized by CNRS, INSERM, INRAe, University’s continuing training courses): (Keywords : basis of microscopy, Advanced training about high resolution microscopy, basis of flow cytometry, FlowJo, basis…
Transmission Electron Microscopy – TEM and Correlative Light Electron Microscopy – CLEM
Our imaging platform has acquired a TEM workstation, which will be installed in July 2025. This new system will open up new possibilities for our users, enabling them to observe…
Transmitted Electron Microscopy: from sample preparation to image analysis
Sample preparation is essential to observe biological samples in electron microscopy. Imagerie-Gif facility propose all the equipment to cover a large diversity of application: chemical fixation, high pressure freezing, resin…
Two photons: calcium and voltage imaging on explants in vivo/ vitro
With the help of the CircuitPhotonics platform we can offer several approaches to imaging living organisms, on brain slices with a TriMscope 1 (LaVision Biotec), in mouse brains with several…
Two-photon microscopy
Ultima 2Plus Bruker, Chameleon vision II Second laser 1000-1300 nm.
Ultrafast in vivo functional optical imaging
Ultrafast in vivo functional optical imaging using AOD two-photon microscopy (brain slices and small animals)
Ultrastructural studies in transmission electron microscopies for plant sciences and animals sciences
Ultrastructural studies in transmission electron microscopies (2D -3D) for plant sciences and animals sciences: from sample preparation (conventional and cryogenic conditions), to imaging & data processing (Key-words: Tokuyasu, Immunolabelling, TEM,…
Unlabelled cellular or tissue imaging: SHG, autofluorescence (kratoscope), Raman, holotomography
Unlabelled imaging can be used to characterise histological sections by mapping them without treatment. It is also possible to complete the analysis using immunohistochemistry, multiplex immunoflorescence or histological staining technologies.…
Volume electron microscopy
Serial sectioning, SBF, FIB-SEM
Volumetric electron microscopy
Volumetric electron microscopy : from sample preparation (chemical fixation), to imaging & data processing (Key-words: SBF, Array-tomography, µCT, micro-wave, nanotomy)
Volumetric electron microscopy for cancer, neurosciences, plants sciences: from sample preparation (conventional and cryogenic conditions), to imaging & data processing
Volumetric electron microscopy for cancer, neurosciences, plants sciences: from sample preparation (conventional and cryogenic conditions), to imaging & data processing (Key-words: HPF, FS, SBF, Array-tomography, FIB SEM, cryoFIB SEM, µCT)
Whole organ, organoids, 3D high resolution imaging and analysis with cell or sub-cellular resolution
High speed, low photobleaching with sub cellular resolution and high detection sensitivity for cleared whole organs, brains or small embryos is offered by the Zeiss light Sheet LS7 -Multiphoton confocal…
Widefield microscopy
Widefield microscopy and deconvolution
2 Widefield microscopes are available for our users. A Leica DMI8 Video-microscope and a Leica Thunder microscope. The Thunder is equiped with 8 Led sources of excitation:390,440,475,510,555,575,635,747 nm. Two Quad…
Workflow volume EM, TOMO TEM, SBF SEM
As Volume EM techniques we offer Electron Tomography and Serial Blockface Scanning Electron Microscopy. The targeting of a precise ROI can be achieved by integrating Light Microscopy or X-ray imaging…
X-ray Microtomography of large samples
X-ray Microtomography of large samples (1mm up to 30cm in diameter) (Key words : 3D volumetric acquisition; large samples; non-invasive inspection; tomodensitometry)