Quantifying Physical parameters (rigidity, tension force production, turgor pressure…) from animal/plant cell to tissue (TFM, Brillouin imaging, photoablation, Triboindenteur, AFM, AFM Hybrid Stage)
Technology: Atomic Force Microscopy (AFM)
A scanning probe technique that maps surfaces at nanometer resolution by moving a sharp tip over the sample, providing topographical and mechanical information.
Atomic force microscopy on living cells or 3D samples is a powerful technique to measure the mechanical properties such as elastic moduli , rigidity, adhesion force, adhesion energy. Combining optical images, AI and AFM, cell population’s mechanome becomes accessible. 3D samples (tissues, organoids) mechanical properties require specific expertise we also provide.
Super-resolution acquisition coupled to high-end atomic force microscopy
Forces (nN range) are applied or probed by atomic force microscopy, while samples are correlatively and simultaneously observed by fluorescence microscopy in time-resolved confocal microscopy, epifluorescence or TIRF. Imaging can be coupled to single molecule localisation superresolution microscopy