nm-characterization of multitypes materials like liposomes, emulsion, hydrogel and porous materials by Cryo-TEM/SEM, and electron tomography,
Technology: Cryo-SEM
SEM imaging performed on frozen samples, preserving delicate biological structures in a near-native state.
We combine latest developments in cryo sample preparation and imaging by electron microscopies (cryo-FIB/SEM and cryo-TEM) to observe biomolecules of interest (ribosomes, carboxysomes, viruses, nuclear pore complexes…) within their cellular or tissular environment. Using cryo-electron tomography followed by image analysis, we are able to determine their 3D structures with near atomic resolution. Altogether, these methodologies pave the way for in situ structural biology studies.
Scanning electron microscopy in cryogenic conditions for plant imaging and soft materials: from sample preparation, to imaging & data processing (Key-words: HPF, plunge freezing, cryofracture, cryoSEM, cryoSEM EDS)