nm-characterization of multitypes materials like liposomes, emulsion, hydrogel and porous materials by Cryo-TEM/SEM, and electron tomography,
Technology: Cryo-TEM
A TEM approach where biological samples are flash-frozen, preserving them close to their natural state without chemical fixation.
We combine latest developments in cryo sample preparation and imaging by electron microscopies (cryo-FIB/SEM and cryo-TEM) to observe biomolecules of interest (ribosomes, carboxysomes, viruses, nuclear pore complexes…) within their cellular or tissular environment. Using cryo-electron tomography followed by image analysis, we are able to determine their 3D structures with near atomic resolution. Altogether, these methodologies pave the way for in situ structural biology studies.
Workflow including sample preparation, 2P microscopy, confocal microscopy, light sheet microscopy, ophthalmic imaging/OCT/ERG and image analysis.
Talos F200X G2
We use Cryo-electron microscopy to capture high-resolution images of frozen molecules, liposomes and other thin specimens, preserving their natural structure.
Multimodal microscopy, by combining fluorescence, harmonic and electron microscopy signals, is therefore of real interest for taking advantage of complementary information and increasing the sensitivity of pathology detection to qualify a complex tissue state.
Ex: Imaging of stimulated Raman scattering (SRS), coherent anti-Stokes Raman scattering (CARS), and two-photon (2P) fluorescence (or autofluorescence). We will thus be able to offer multimodal analysis on a single sample section in order to obtain spatial mapping of molecular compounds of interest, such as CH2 groups for lipid analysis (steatosis assessment) or CH3 for protein analysis.