nm-characterization of multitypes materials like liposomes, emulsion, hydrogel and porous materials by Cryo-TEM/SEM, and electron tomography,
Technology: Electron tomography
Combines TEM images taken at different tilt angles to reconstruct detailed 3D views of complex structures.
We combine latest developments in cryo sample preparation and imaging by electron microscopies (cryo-FIB/SEM and cryo-TEM) to observe biomolecules of interest (ribosomes, carboxysomes, viruses, nuclear pore complexes…) within their cellular or tissular environment. Using cryo-electron tomography followed by image analysis, we are able to determine their 3D structures with near atomic resolution. Altogether, these methodologies pave the way for in situ structural biology studies.
Correlative light/electron microscopy (CLEM)
3D cellular or tissue imaging by electron microscopy (SerialBlock Face SEM and thick sections in electron tomography)
Cryo-ET of in vitro reconstituted samples, extracellular vesicles, and organelles OR electron tomography at room temperature of thick sample (300 nm)
As Volume EM techniques we offer Electron Tomography and Serial Blockface Scanning Electron Microscopy. The targeting of a precise ROI can be achieved by integrating Light Microscopy or X-ray imaging in the pipeline.
Ultrastructural studies in transmission electron microscopies (2D -3D) for plant sciences and animals sciences: from sample preparation (conventional and cryogenic conditions), to imaging & data processing
(Key-words: Tokuyasu, Immunolabelling, TEM, SBF, tomography, 3D reconstructions, segmentation, image analysis)