La personne recrutée rejoindra une équipe de 5 ingénieurs qui assure le fonctionnement et le développement de la plateforme de microscopie photonique de l’IGBMC pour répondre aux besoins multidisciplinaires des chercheurs de l’institut et des unités de recherche de l’université de Strasbourg. Parmi les plates-formes scientifiques, la plate-forme de microscopie photonique accueille 35 équipes de recherche de l’IGBMC, 8 équipes externes (académiques et industriels), ~200 utilisateurs/an au total.

Mission 

Pour répondre à des besoins multidisciplinaires, la plate-forme de microscopie  photonique de l’IGBMC labélisée IBiSA et composante du Nœud Alsace de France-BioImaging dispose de 15 systèmes optiques (microscopes champ large, confocaux, multiphoton, spinning disk, microscope à feuille de lumière, macroscopes, microdissecteur laser, techniques de type FRET, FRAP, et des stations de travail pour le traitement et l’analyse des données. Au sein de cette plateforme, l’ingénieur(e) interagira avec les membres du Centre d’Imagerie et avec les utilisateurs du service (équipes IGBMC et utilisateurs extérieurs), Il/elle participera à la formation et à l’assistance des utilisateurs dans leur pratique de la microscopie photonique, à la maintenance des systèmes et au développement de nouvelles approches d’imagerie avec l’équipe de la plateforme.

Activités principales 

  • Assurer la formation des utilisateurs sur les différents systèmes de la plate-forme de microscopie photonique
  • Assister, guider et autonomiser les utilisateurs dans leur pratique de la microscopie en lien avec leurs projets de recherche ; connaitre les limites et les possibilités des techniques proposées.
  • Contribuer à la maintenance préventive et détecter les dysfonctionnements des systèmes.

Activités associées 

  • Participer aux enseignements et formations/ateliers pratiques organisés par le centre d’imagerie.
  • Participer à des réunions dans le cadre des réseaux d’imagerie nationaux et internationaux ; participer à des conférences ateliers thématiques
  • Optimiser les protocoles et de marquage des échantillons en adéquation avec la technique d’imagerie cible.

Compétences

Savoir sur l’environnement professionnel 
  • Connaissances théoriques et pratique approfondies l’imagerie photonique multidimensionnelle (microscopie time-lapse à champ large, confocale, biphotonique, à feuille de lumière).
  • Connaissance en biologie cellulaire, tissulaire et du développement
  • Une expérience dans les techniques de préparations des échantillons pour l’imagerie serait un plus.
 Savoir-faire opérationnel
  • Bon sens relationnel avec de sérieuses qualités pédagogiques, d’écoute et de communication devant s’exercer dans le cadre d’un service commun
  • Motivation pour l’activité en plate-forme technique
  • Maitrise de l’anglais lu/parlé (niveau 1) indispensable.
Savoir-faire comportemental
  • Prise d’initiative et curiosité
  • Autonomie
  • Sens du travail en équipe et organisationnel.

 

Contact pour renseignements sur le poste et pour postuler, adressez votre CV et lettre de motivation au plus tard le 23/03/2023 à l’adresse suivante : Bertrand VERNAY,  Responsable de la plateforme

Mail : vernayb@igbmc.fr ; Tél : +33 (0)3 69 48 51 27

We are looking for a bio-image analyst to join our light microscopy facility team at the Institute of Genetics and Molecular and Cellular Biology.

Our institute
The Institute brings together nearly 600 research staff divided into 4 scientific departments dedicated to research in genetics, developmental and structural biology, and translational medicine. With its high-level equipment and scientific platforms, the Institute also offers a quality environment for the training of young researchers. A fertile ground for creativity and knowledge, the research carried out at the Institute has regularly led to numerous publications in leading scientific journals and many innovations have emerged, notably through the creation of several start-ups.

Our facility
The light microscopy imaging facility of the IGBMC and its 5 team members insure the access of researchers of the broad scientific community to a wide range of high-end photonic systems and techniques: widefield, confocal, multiphoton, spinning disk, lightsheet and laser micro dissection microscopes. The expertise of the 5 team members comprises physics, cell and developmental biology, optics and instrumentation.

Other imaging techniques are also available at the IGBMC technological facilities and do require bio-images analysis: cellular electron microscopy (CLEM), high-content screening, optical projection tomography, high-resolution episcopic microscopy, whole-slide scanner.

The analyst missions are to

  • develop a service of bio-images processing and analysis and data management for the light microscopy facility (and cellular electron microscopy and high content screening)
  • develop bio-images processing and analysis pipelines/tools for the researchers and the facility staff
  • participate to the program of bio-images processing and analysis workshops for the institute’s staff

More info at: here or by directly contacting the facility manager Bertrand Vernay vernayb@igbmc.fr

Working language: French & English
Contrat duration: from start of employement till 31/08/2022, initially renewable for a year (till 31/08/2023).
Diploma required: doctorate degree or national diploma of engineer

Best wishes

Bertrand

To contact the facility: groupe-mic-photon@igbmc.fr


Bertrand Vernay, PhD
Head of the Photonic Microscopy Facility
http://quest.igbmc.fr/
http://www.igbmc.fr/technologies/5/team/55/

Office:+33 (0)3 69 48 51 27
Office: CBI, E1004
ORCID 0000-0002-7843-3872
IGBMC – CNRS UMR 7104 – Inserm U 1258
1 rue Laurent Fries
BP 10142
67404 Illkirch CEDEX
France Tél +33 (0)3 88 65 32 00
Fax +33 (0)3 88 65 32 01

At the Bordeaux Imaging Center (BIC), we are looking for a motivated cell biologist to implement sample preparation, super-resolution imaging in cryogenic conditions and image treatment and analysis.

Cryo-electron microscopy provides not only access to the structure of isolated molecules at an atomic scale, but also to their structure in a cellular context. The need of a specific and highly precise localization of molecules previous to cryo-EM observation in a frozen hydrated sample has become evident. Super-resolution microscopy techniques based on single molecule localization could overcome the challenge of nanoscale detection in light microscopy. The EquipEx+ project NanoCryoCLEM coordinated by the University of Bordeaux aims to establish a cryo-CLEM workflow for super-resolution microscopy. In this context, the Bordeaux Imaging Center (BIC), the Institute interdisciplinaire de Neurosciences (IINS), and the Institute d’optique (LP2N), will collaborate to develop a new super-resolution optical system in cryogenic conditions. The ultimate goal of this project is the transfer of these frozen hydrated samples, analyzed by super-resolution microscopy, to the electronic microscope from the Institute Européen de Chimie et Biologie (IECB) for Cryo-tomography observation and structural analysis.

The main activities of the candidate will include:

  • To develop new protein labeling strategies with probes compatible for both optical super-resolution microscopy techniques, and protein localization in cryo-electron tomography. This work will be carried out in collaboration with research teams from IINS: «Spatio-Temporal and Mechanical Control of Motile Structures» led by Grégory Giannone, and «Dynamic Organization and Function of Synapses» led by Daniel Choquet.
  • To establish cell cultures on EM grids preserving specific subcellular structures and to plunge freeze the samples.
  • To acquire super-resolution images in cryogenic conditions with a home-made system.
  • To analyze and reconstruct super-resolved images.
  • To develop a correlative workflow in order to select and localize regions of interest in the optical system for cryo-electron tomography acquisition.

The candidate must hold a PhD and should have advanced expertise in cell biology and fluorescence microscopy techniques. In-depth knowledge of protein labeling biochemistry in cell cultures will be an advantage. Previous experience in cryo-fixation techniques and liquid nitrogen manipulation will also be an asset.

The candidate will be working in an interdisciplinary environment, thus strong teamworking skills and fluid English will be asked. The contract is 1 year research engineer renewable up to 3 years, salary depending on CV.

To apply please send a full CV and a cover letter to:

  • Mónica Fernández Monreal (monica.fernandez-monreal AT u-bordeaux.fr).

  • Gregory Giannone (gregory.giannone AT u-bordeaux.fr).

  • Daniel Choquet (daniel.choquet AT u-bordeaux.fr).

About us

CryoCapCell is a biotech start-up that conceives and develops innovative tools to prepare biological specimen for correlative light and electron microscopy analysis.

Based at the Kremlin-Bicêtre hospital, near Paris (metroline 7, Le Kremlin-Bicêtre) in recently refurbished lab and office spaces, we interact with scientists and medical doctors in a very dynamic environment to address scientific and medical questions.

We offer a friendly and very dynamic environment and are supportive to continuous education and external training.

The opportunity

Our company grows and expands its activity. We are looking for a motivated scientist to conduct R&D bench experiments around live-cell correlative High-Pressure Freezing (HPM Live µ) and the subsequent embedding step by freeze-substitution (developments on our exclusive R221 electron conductive resin), electron microscopy and image registration.

At first, you will get familiar with our technologies and research projects. Then you will progressively develop services to external scientists, from project identification to results delivery in direct contact with the customers.

To conduct the mission, we own a broad range of equipment, from live cell imaging to electron microscopy and have access to state-of-the-art facilities in Paris, or through international collaborations.

About you

The Staff scientist / project manager will have the following skills and attributes:

  • Qualified in a biological science from B.Sc. up to Ph.D. involving a significant microscopy component or equivalent practical experience (minimum 3 years) is essential
  • Experience in cell culture (basics)
  • Experience in optical and electron microscopy : sample preparation, analysis and interpretation of results (advanced supervision will be proposed)
  • Interest in using and developing correlative imaging workflows
  • A good understanding of a diverse range of applications for electron microscopy
  • A desire to handle external project with a certain degree of freedom (adapted to the scientist level and wish) in contact with scientists.
  • Working knowledge of other microscopy techniques such as confocal, fluorescence, AFM, Raman spectroscopy or others will be regarded as advantageous
  • Excellent problem solving skills and able to clearly articulate complex, technical issues. (essential)
  • Organized, with solid administrative/reporting skills and proficient informatics skills including MS Office
  • French is not a prerequisite if English is mastered
  • Interest in supporting the development of training materials, technical documentation and sales collateral (optional)
  • Interest in attending (physical and virtual) international conferences to maintain your expertise at international standard

Key responsibilities for the staff scientist / project manager

  • Your primary responsibility will be as staff scientist / project manager to run CLEM experiments according to each project, including:
    • sample culture
    • live imaging
    • sample vitrification / sample fixation
    • electron microscopy preparation
    • in-resin fluorescence imaging
    • electron microscopy (SEM/TEM/STEM) imaging
    • multimodal image registration
    • formal experimental reporting
  • Test and establish appropriate protocols for the newly developed technologies
  • Welcome visiting scientists/customers and conduct their experiments
  • Keep track of all projects and report to customers on progresses
  • Organize daily access to equipment and report defect to anticipate maintenance
  • Work with the business manager to identify potential customers and propose adapted business strategy
  • Work with the business manager to identify technological development opportunities
  • Participate in remote demonstrations of our technologies

Benefits for the lab manager

In addition to a competitive starting salary, we offer structured career development opportunities and a good work-life balance. As an example, we encourage remote working when appropriate. Finally, CryoCapCell offers 25 days annual leave, covers 50% of health insurance and 50% of public transport travel card.

The successful candidate will be given all the necessary training and support and they will acquire skills in both sample preparation, sample transfer, microscope alignment and maintenance (both from X-ray and fluorescence imaging), data collection and image correlation. All this while using and developing novel cutting-edge technology and helping our user base to bring about excellent research results.

 

This post would be ideal as a stepping stone for career development for any interested party that has completed a relevant undergraduate degree or further. They will need to have had exposure to biological laboratory procedures and microscopy methods.

We are seeking to recruit a postdoctoral fellow to develop and apply high resolution light sheet microscopy in order to image cytoskeletal networks and adhesion complexes in developing embryos (e.g. Drosophila). We have recently developed a light sheet microscope for fast 3D imaging and we aim at including a new illumination scheme to achieve higher resolution and single molecule detection. The recruited postdoc will also develop new image analysis tools to analyze the generated high resolution data.

The candidates should have experience in computational image analysis and/or optical engineering (including software engineering for machine control). The postdoc will benefit from an interdisciplinary environment with expertise in imaging, optical engineering, physics and cell developmental biology (Labex Inform, IBDM) The postdoctoral fellowship is offered for a period of two years.

Applicants should send a CV, names of two referees, and a short outline of their research interests to P.-F. Lenne and T. Lecuit.

Member of C2RT (Center for Resources and Research in Technology) the Unit for service and technology in ultra-structural bio-imaging  (UBI) at Pasteur provides technical and scientific support in electron microscopy, mainly, but not exclusively, to the Pasteur community, analyzing host-pathogen interactions at the ultra-structural level. Our equipment enables us to do state-of-the-art EM for life sciences that includes three dimensional EM by tomography and focused ion beam SEM. The ultrapole is run by ten members (eight engineers and two technicians), each specialized in certain techniques. We put a big emphasis on correlate light- and electron microscopy to study key (rare events) of host-pathogen interactions and we have designed several protocols to combine room temperature LM and cryo-LM with FIB-SEM, cryo-SEM and cryo-tomography.

The UBI also has a small research group focusing on large DNA-viruses. Through this research theme we wish to establish and develop robust, new protocols for various EM-techniques.

The cell biology pole Imagerie-Gif is localized on the CNRS campus of Gif sur Yvette, in a new building dedicated to platform activity. This IBiSA platform provides efficient access to high quality services and state of the art technologies. It is open to the whole academic scientific community and to industrial partners. The management and development of this pole is under the responsibility of the team “Dynamics of cell compartmentation” (group leader S. Lecart, Institut des Sciences du Végétal, Gif sur Yvette). It uses cell biology approaches and multiscale imaging (cytometry, bio-imaging and electron microscopy) to explore the cell. The development of new protocols and the mastering of update imaging approaches are part of the R & D objectives of the platform. Those are then transferred to platform services and disseminated through numerous training and teaching events, and opened to the whole scientific community. The cell biology pole of Imagerie-Gif activities contributes to the working groups “super resolution”, “Probe development” and “CLEM” within the France-BioImaging consortium.

Microscopy systems available @Imagerie-Gif

Montpellier Ressources Imagerie (MRI) is a distributed imaging facility present on six sites in Montpellier (www.mri.cnrs.fr). MRI is labeled IBiSA and certified ISO9001-2008 LQRA. It has a staff of 30 engineers and is directed by P. Lemaire (CNRS). MRI manages numerous microscopes (36 photonic and 2 electron microscopes) and 14 analysis workstations, and especially microscopes for long term or short live experiments. MRI offers a complete set of state-of-the-art technologies, from single molecule to small organism imaging. The platform offers and develops 3D-SIM, SPIM, FCS/FCCS, CLEM and 2photons microscopies, and also develops a new service of High Content Screening, with a specific emphasis on gene expression analysis by smFISH techniques. MRI organizes regular training sessions with theoretical presentations and practical sessions about advanced light microscopy and image analysis. Once trained, a user can freely access microscopes on a pay-per-use basis. For the screening facility, the access is evaluated on a project-by-project basis.

Microscopy systems available @MRI

The BIC (Bordeaux Imaging Center) offers resources in photonic and electronic imaging, mainly in life, health and plant sciences. It is a core facility identified at the national level as IBISA that gathers 12 highly skilled engineers. It has the ISO9001 label. The different components of the BIC are: PHOTONIC imaging, ELECTRONIC imaging, PLANT imaging. The Bordeaux Imaging Center offers access to the most advanced bio-imaging techniques for fixed and live cell imaging such as video-microscopy, confocal microscopy, multiphoton microscopy, transmission electron microscopy and scanning electron microscopy. The BIC provides a unique set of high-end equipment for super- resolution microscopy such as STED confocal microscopy, FRAP video-microscopy, lifetime imaging FLIM for the measurement of molecular interactions. We also provide access to equipment for sample preparation such as ultra-microtoms, high pressure freeze (HPF) and we can host live samples.

Microscopy systems available @BIC

The Cell and Tissue Imaging Platform (PICT-IBiSA) of the Institut Curie brings together advanced microscopy technologies. Its main objective is to provide researchers in cell biology, development, structural, chemistry and biophysics with imaging approaches at different spatial and dynamic scales ranging from the molecule, to the cell, to the organism in healthy or pathological contexts.

The platform is organized around 3 poles: photonic microscopy, high-content screening, electron microscopy and cryo-electron microscopy. Photonic microscopy extends from dynamic imaging to high resolution. High throughput microscopy allows cellular screening of chemical and siRNA banks. Electron microscopy and cryo-microscopy provide the molecular structure and cellular ultrastructure of biological samples. The platform also offers expertise in data processing and analysis.

ImagoSeine imaging facility gathers advanced light microscopy, cytometry and electronic microscopy activities, installed on 450 m² at IJM. Rooms for cells and tissues manipulations are provided to external users. ImagoSeine has been assessed and found to meet the requirements of ISO 9001 by AFNOR Certification. ImagoSeine brings together technologies and expertise in these fields, thanks to the 7 permanent and 2 contractual engineers. The originality of ImagoSeine-IJM is the close collaboration between the imaging facility which have a long experience of making available standard approaches but also new developments to the biological community and a research team involved in the development of state of the art functional imaging technologies (FLIM-FRET; FCCS) and more recently superresolution and non-linear microscopy. The ultimate goal is to provide access and training to these emerging techniques and methods for the realization of competitive biological projects.