Multiscale imaging of complex 3D objects: from embryonic to subcellular imaging (adaptive confocal and two-photon microscopy, light-sheet imaging of cleared tissues; NIR detection of luminescent probes, IA-based image analysis)
Technology: Light-sheet microscopy
The sample is illuminated by a thin sheet of light from the side, which reduces photodamage and allows rapid 3D imaging. It is particularly well suited for observing large, living specimens.
To decipher Inflammatory and cardiovascular diseases, multiplex imaging and cytometry pipelines include the entire process from sample preparation, image acquisition to image analysis and quantification. Our workflows are adpated to mouse or human lymphoid organs and blood . They employ techniques such as confocal spectral imaging and cytometry-based analysis (spectral, sorting, and analysis).
To tackle complex biological questions in aging and geroscience, we design and implement advanced multimodal, multiparametric, and multiscale pipelines in imaging, flow cytometry, and image flow cytometry. Our workflows span the entire process—from sample preparation to data acquisition and multidimensional analysis. We investigate biological systems across multiple scales, from subcellular structures such as mitochondria to organoids, whole organs, and even entire organisms. Our pipelines are tailored to a wide range of tissues, including adipose tissue, muscle, bone marrow, skin, blood, and periodontal tissue. In addition, our platforms provide access to complementary techniques such as second- and third-harmonic generation (SHG, THG), spectral confocal imaging, high-content imaging, image-based cytometry, conventional and spectral flow cytometry, and mechanobiological analysis. These integrative approaches are designed to support comparative and translational research across multiple species, including fish (with an emphasis on the African turquoise killifish as a model of aging), drosophila, mice, and humans.
Organoids (lung, endometriosis, digestive tract, adipose tissue, bone marrow, skin, bladder, fallopian tube): integrative analysis (sample preparation, acquisition – light sheet, confocal, two-photon, high-content screening, multiparametric analysis, 3D imaging, analyses, flow cytometry and imaging)
Light-sheet imaging of fixed and live samples. We can afford (cells, IPS, organoïds, hydra, fish, human tissues) because the microscope is installed in Biology Safety Level 2
Cell-biology expertise: providing cells(epithelial, glial, neuronal) and organoïds, labelling (transfections or probe-Test), and imaging for physicist, chemist that would like to test a prototype. Including : Guidance on super-resolution modalities (collaboration) : from system choice (SIM-STED-STORM) to images processing and data analysis (tools development).
Full pipeline support on light sheet and biphoton imaging (from sample prep and tissue clearing to imaging)
On demand from basic training for image acquisition to full pipeline support in multi modal imaging
Numerous tests to monitor the performance of photonic microscopy systems, including light source power, observation field homogeneity, detector stability, etc. Training at PRIMACEN or at the applicants’ site.
Workflow including sample preparation, 2P microscopy, confocal microscopy, light sheet microscopy, ophthalmic imaging/OCT/ERG and image analysis.
Workflow including sample preparation, light sheet imaging, and fluorescence macroscopy
Workflow including sample preparation, light sheet microscopy, confocal microscopy, intravital microscopy, and image analysis.