Numerous tests to monitor the performance of photonic microscopy systems, including light source power, observation field homogeneity, detector stability, etc. Training at PRIMACEN or at the applicants’ site.

Workflow including sample preparation, light sheet imaging, and fluorescence macroscopy

In vivo visualization of plant tissues cellular development, as well as of their response to climate variations
(Key-words : PICPLANT facility with greenhouses (S2), 3 growth chambers for small and large plants, CONVIRON climatic chamber, histology, stereomicroscope, Spectral-FLIM confocal microscope.

Mono and bi-photon longitudinal imaging with flexible windows and/or stabilizing chambers for imaging peripheral organs (e.g. heart, lung, liver, gut, pancreas) and human organoids/spheroids/tumoroids implanted in mouse or zebrafish models. This provides information about how they get connected to the blood vasculature.

Intravital mono or bi-photon imaging with either short or long (up to 2 cm) working-distance objectives and coupled with cellular manipulation (mouse, fish and insect models). This can be applied to high resolution cellular imaging of molecule extravasation (e.g. nanobodies, drugs, hormones), capillary blood flow, cellular migration and signaling (e.g. calcium, ligand sensors), and molecule distribution into the extracellular space.

Acquisition of images using confocal/multiphoton macroscopes/microscopes in dorsal chambers or on surgically exposed organs (without chamber).

Multi-modality dorsal chamber (photonics, MRI, ultrasound) enabling long-term longitudinal monitoring (several weeks). Performance (in collaboration or team training) of surgical transplants and high-resolution image acquisition using macroscopes/confocal/multiphoton microscopes.

the imaging system Kratoscope acquires an image of the organ in the block of tissue each time a slice is cut by a microtome (FFPE blocks) or a cryostat (frozen tissue), in a fully automated process. By getting a stack of slices at increasing depth, it is then possible to reconstruct a 3D view of the organ.
In addition to a possible fluorescent signal detection, the contrast of the images comes from the difference in autofluorescence of different structures of the organ and highlights anatomical its anatomy. It can provide information not visible on micro CT images, when small structures lack radio contrast.

support from sample preparation to image analysis – including visualisation of organoids/spheroids in 3D imaging. Pool of systems and expertise for these studies (transparization, staining, light sheets, etc.).

The Imaging Facility at IBMP-CNRS provides researchers with access to state-of-the-art microscopy techniques, including fluorescence, confocal, and 3D scanning electron microscopy. The platform supports a variety of sample preparation workflows, such as live-cell imaging and cryotechniques, and offers guidance at every step, from experimental design to image data analysis. Services are available for projects on plant tissues, microbial systems, and cell cultures, enabling users to visualize structures and processes at high resolution. The facility is dedicated to making advanced imaging approaches accessible, fostering collaboration, and helping researchers obtain reliable, quantitative results.