Technology: Molecular Interaction based microscopy (FRET, FLIM, FCS, FRAP)
A group of fluorescence techniques that go beyond structural imaging to study how molecules interact, move, and behave inside living cells, providing insight into their dynamics and functions.
Imaging, quantifiying and controlling cell metabolism (FLIM, TIRF-FastFLIM, ratipmetric-SD, optogenetis…)
Multiscale measurements of molecular dynamics in cell and tissue (SPT, FCS, tICS, AiryScan, SIM, STED, SMLM, , Live SR, FRAP, live confocal imaging, Live TIRF and optogenetics)
Quantitative FRET and stoichiometry measurements to map biological activity in cells : fluorescence quantification via an universal QuanTI-FRET protocol
Quantification of density and structure of biofunctional surfaces (FRAP and reflection interference contrast microscopy-RICM) and nanometric dynamic cell-substrate distance mapping
STED correlative imaging with molecular dynamic and interaction (FCS-FLIM-FRAP-FRET) on thin, thick samples
Cell-biology expertise: providing cells(epithelial, glial, neuronal) and organoïds, labelling (transfections or probe-Test), and imaging for physicist, chemist that would like to test a prototype. Including : Guidance on super-resolution modalities (collaboration) : from system choice (SIM-STED-STORM) to images processing and data analysis (tools development).
Functional microscopy at the molecular, cellular, and tissue levels (FLIM-FCS, FRET, FRAP, laser ablation)
On demand from basic training for image acquisition to full pipeline support in multi modal imaging
Numerous tests to monitor the performance of photonic microscopy systems, including light source power, observation field homogeneity, detector stability, etc. Training at PRIMACEN or at the applicants’ site.
Workflow from sample preparation, MET and cryo-confocal, advanced photonic microscopy
WF/confocal/FLIM, CLEM, image analysis (AMIRA)