Gene expression, DNA damage, DNA structure and DNA-binding proteins structures, super-resolution (SMLM, RIM, SIM, Airyscan), single particle tracking, photo-manipulation, Correlative Light Electron Microscopy (CLEM)
Technology: Photon counting microscopy
This highly sensitive method detects individual photons of light, improving the precision of measurements in very low-light conditions, often used in fluorescence lifetime or super-resolution techniques.
Maintain of the mouse living organs under 2P microscope Bruker 2P+, thanks to perfusion and oxygenated medium
Numerous tests to monitor the performance of photonic microscopy systems, including light source power, observation field homogeneity, detector stability, etc. Training at PRIMACEN or at the applicants’ site.
Workflow from sample preparation, MET and cryo-confocal, advanced photonic microscopy
WF/confocal/FLIM, CLEM, image analysis (AMIRA)
Stellaris 8, WLL, FALCON, Leica Microsystems
Stellaris 8, WLL, FALCON, 592 and 775 nm for depletion, Leica Microsystems
Forces (nN range) are applied or probed by atomic force microscopy, while samples are correlatively and simultaneously observed by fluorescence microscopy in time-resolved confocal microscopy, epifluorescence or TIRF. Imaging can be coupled to single molecule localisation superresolution microscopy
Measuring biomolecular structure, dynamics and interactions in vitro and in living cells. This includes distances and interactions measurements by single molecule FRET and FRET/FLIM and interactions and dynamics by FCS
We use homemade microscope dedicated with high sensitivity and Z scan to choose the right focus on cells compartments
Strong local expertise in multi-contrast multiphoton tissue imaging based on 2PEF, 3PEF, SHG, 3PEF, polarization and ZP-FLIM contrasts. Acces to lab-built and application-oriented systems for pilot studies after a project evaluation by our scientific commitee.
Light microscopy enables biosensors to be used to characterize the cellular environment (pH, ROS, calcium, etc.) in order to highlight differences between populations or identify heterogeneities within tissues or cells themselves. Cytometry allows multiparametric analyzes offering the possibility of measuring several activities simultaneously cell by cell. It is thus easy to compare the results obtained, to deduce their interdependence or not, which makes it an essential tool for the study of cell signaling.
In order to characterise dynamic phenomena at the molecular level, we have developed expertise in evaluating their diffusion (FRAP) and highlighting interactions (FRAP/FRET-FLIM).