Inflammatory and cardiovascular diseases are complex, multifactorial conditions that affect multiple organs and cellular components. To tackle these intricate biological challenges, we employ and advance multi-modal and multi-scale imaging and flow cytometry pipelines. These pipelines include the entire process from sample preparation, image acquisition to image analysis and quantification. Our workflows are adpated to various tissues, including the heart, kidneys, blood vessels, muscles, liver and adipose tissue. They operate across scales ranging from whole organs to subcellular levels, employing techniques such as widefield imaging, confocal spectral imaging, high-resolution imaging (Airyscan), high-content imaging (Axioscan 7, Opera Phenix), and cytometry-based analysis (spectral, sorting, and analysis).
Technology: Polarized light microscopy
By using polarized light, this technique reveals structures with specific optical properties, such as crystals, fibers, or ordered biological molecules. It is especially useful for studying tissues like muscle or collagen.
2 Widefield microscopes are available for our users. A Leica DMI8 Video-microscope and a Leica Thunder microscope. The Thunder is equiped with 8 Led sources of excitation:390,440,475,510,555,575,635,747 nm. Two Quad filters to spectrally separate the 8 channels on the following filters: 420-450; 462-484; 506-532; 527-551; 578-610; 602-680; 666-724; 770-850.
Strong local expertise in multi-contrast multiphoton tissue imaging based on 2PEF, 3PEF, SHG, 3PEF, polarization and ZP-FLIM contrasts. Acces to lab-built and application-oriented systems for pilot studies after a project evaluation by our scientific commitee.