Numerous tests to monitor the performance of photonic microscopy systems, including light source power, observation field homogeneity, detector stability, etc. Training at PRIMACEN or at the applicants’ site.
Technology: Stereomicroscopy
A binocular optical technique that provides 3D views of specimens at low magnification.
Mono and bi-photon longitudinal imaging with flexible windows and/or stabilizing chambers for imaging peripheral organs (e.g. heart, lung, liver, gut, pancreas) and human organoids/spheroids/tumoroids implanted in mouse or zebrafish models. This provides information about how they get connected to the blood vasculature.
Intravital mono or bi-photon imaging with either short or long (up to 2 cm) working-distance objectives and coupled with cellular manipulation (mouse, fish and insect models). This can be applied to high resolution cellular imaging of molecule extravasation (e.g. nanobodies, drugs, hormones), capillary blood flow, cellular migration and signaling (e.g. calcium, ligand sensors), and molecule distribution into the extracellular space.