Multiscale measurements of molecular dynamics in cell and tissue (SPT, FCS, tICS, AiryScan, SIM, STED, SMLM, , Live SR, FRAP, live confocal imaging, Live TIRF and optogenetics)

Gene expression, DNA damage, DNA structure and DNA-binding proteins structures, super-resolution (SMLM, RIM, SIM, Airyscan), single particle tracking, photo-manipulation, Correlative Light Electron Microscopy (CLEM)

T-cell cytotoxic function relies on the cooperation between the highly specific T-cell receptor (TCR) and integrins of co-stimulatory molecule at the immunological synapse. Super resolution 3D STED (Leica SP8, 775 nm) is used to study the oorganisation of these molecules during the activation of immune response. Image cytometer (ImageSteam) allows the quantification of molecules recruited at the immunological synapse in a large number of cells.

STED correlative imaging with molecular dynamic and interaction (FCS-FLIM-FRAP-FRET) on thin, thick samples

Low signal fast live imaging at nano-scale level (Airy / Fast Airyscan , Live-3D-STED, Live-3D-SLMN)

Cell-biology expertise: providing cells(epithelial, glial, neuronal) and organoïds, labelling (transfections or probe-Test), and imaging for physicist, chemist that would like to test a prototype. Including : Guidance on super-resolution modalities (collaboration) : from system choice (SIM-STED-STORM) to images processing and data analysis (tools development).

:STED-FLIM BSL-2 : Live / 3D / multi-labeling

Airyscan, SIM, STORM modes

Correlation microscopy between super-resolution acquisition and collagen harmonic signalling

Super-resolution acquisition coupled to high-end atomic force microscopy

On demand from basic training for image acquisition to full pipeline support in multi modal imaging

Full pipeline support on single molecule microscopy equipment (from sample to data analysis)