Gene expression, DNA damage, DNA structure and DNA-binding proteins structures, super-resolution (SMLM, RIM, SIM, Airyscan), single particle tracking, photo-manipulation, Correlative Light Electron Microscopy (CLEM)

Atomic force microscopy on living cells or 3D samples is a powerful technique to measure the mechanical properties such as elastic moduli , rigidity, adhesion force, adhesion energy. Combining optical images, AI and AFM, cell population’s mechanome becomes accessible. 3D samples (tissues, organoids) mechanical properties require specific expertise we also provide.

Bruker 2P+, Discovery laser (680 to 1340 nm), mouse anesthesy and stabulation. Cleared mouse organs (gut, lymph nodes..) and 2P microscopy

To decipher Inflammatory and cardiovascular diseases, multiplex imaging and cytometry pipelines include the entire process from sample preparation, image acquisition to image analysis and quantification. Our workflows are adpated to mouse or human lymphoid organs and blood . They employ techniques such as confocal spectral imaging and cytometry-based analysis (spectral, sorting, and analysis).

To tackle complex biological questions in aging and geroscience, we design and implement advanced multimodal, multiparametric, and multiscale pipelines in imaging, flow cytometry, and image flow cytometry. Our workflows span the entire process—from sample preparation to data acquisition and multidimensional analysis. We investigate biological systems across multiple scales, from subcellular structures such as mitochondria to organoids, whole organs, and even entire organisms. Our pipelines are tailored to a wide range of tissues, including adipose tissue, muscle, bone marrow, skin, blood, and periodontal tissue. In addition, our platforms provide access to complementary techniques such as second- and third-harmonic generation (SHG, THG), spectral confocal imaging, high-content imaging, image-based cytometry, conventional and spectral flow cytometry, and mechanobiological analysis. These integrative approaches are designed to support comparative and translational research across multiple species, including fish (with an emphasis on the African turquoise killifish as a model of aging), drosophila, mice, and humans.

Inflammatory and cardiovascular diseases are complex, multifactorial conditions that affect multiple organs and cellular components. To tackle these intricate biological challenges, we employ and advance multi-modal and multi-scale imaging and flow cytometry pipelines. These pipelines include the entire process from sample preparation, image acquisition to image analysis and quantification. Our workflows are adpated to various tissues, including the heart, kidneys, blood vessels, muscles, liver and adipose tissue. They operate across scales ranging from whole organs to subcellular levels, employing techniques such as widefield imaging, confocal spectral imaging, high-resolution imaging (Airyscan), high-content imaging (Axioscan 7, Opera Phenix), and cytometry-based analysis (spectral, sorting, and analysis).

Organoids (lung, endometriosis, digestive tract, adipose tissue, bone marrow, skin, bladder, fallopian tube): integrative analysis (sample preparation, acquisition – light sheet, confocal, two-photon, high-content screening, multiparametric analysis, 3D imaging, analyses, flow cytometry and imaging)

Full pipeline support for single molecule RNA FISH

Numerous tests to monitor the performance of photonic microscopy systems, including light source power, observation field homogeneity, detector stability, etc. Training at PRIMACEN or at the applicants’ site.

Workflow including sample preparation, 2P microscopy, confocal microscopy, light sheet microscopy, ophthalmic imaging/OCT/ERG and image analysis.

Workflow including sample preparation, light sheet microscopy, confocal microscopy, intravital microscopy, and image analysis.

Workflow from sample preparation, MET and cryo-confocal, advanced photonic microscopy
WF/confocal/FLIM, CLEM, image analysis (AMIRA)