2D or 3D electron microscopy morphometric analysis of the central nervous system with Deep FIB-SEM and IA-based image analysis

nm-characterization of multitypes materials like liposomes, emulsion, hydrogel and porous materials by Cryo-TEM/SEM, and electron tomography,

Connecting different resolutions for correlative confocal/SEM and confocal/TEM on adherent cells

Imaging, quantifiying and controlling cell metabolism (FLIM, TIRF-FastFLIM, ratipmetric-SD, optogenetis…)

Quantitative FRET and stoichiometry measurements to map biological activity in cells : fluorescence quantification via an universal QuanTI-FRET protocol

Quantification of density and structure of biofunctional surfaces (FRAP and reflection interference contrast microscopy-RICM) and nanometric dynamic cell-substrate distance mapping

Quantitative measurement of intracellular forces on living cells, but also within tissues (Drosophila, pupae, mouse intestines). Mechanotransduction, micro-nanorheology. High-resolution fluorescence imaging using remote focusing coupled with force measurements.
Compression force measurements

T-cell cytotoxic function relies on the cooperation between the highly specific T-cell receptor (TCR) and integrins of co-stimulatory molecule at the immunological synapse. Super resolution 3D STED (Leica SP8, 775 nm) is used to study the oorganisation of these molecules during the activation of immune response. Image cytometer (ImageSteam) allows the quantification of molecules recruited at the immunological synapse in a large number of cells.

To decipher Inflammatory and cardiovascular diseases, multiplex imaging and cytometry pipelines include the entire process from sample preparation, image acquisition to image analysis and quantification. Our workflows are adpated to mouse or human lymphoid organs and blood . They employ techniques such as confocal spectral imaging and cytometry-based analysis (spectral, sorting, and analysis).