Full pipeline support on light sheet and biphoton imaging (from sample prep and tissue clearing to imaging)

Live Imaging and molecular dynamics for cell, organoids, tissue and embryo : from sample preparation with access to a L2 cell culture room, to microscopy and data analysis
(Key words : spinning disk, lattice light sheet, widefield, LightSheet, FRAP, photoconversion, FCS, spot variation FCS, TIRF, single molecule, FRET, FLIM))

Holotomography merges holography and tomography to solve the challenge of recovering light phase shifts to generate image contrast in Quantitative Phase Imaging. HT illuminates a cell with very low power visible light at various illumination angles and measures the phase delay of the transmitted light. Holotomography’s advantages lie in its capability to provide non-invasive, label-free 3D images of live cells, allowing real-time studies while the measurement of RI tomogram also allows quantitative analysis. Its data acquisition speed surpasses traditional methods, making it invaluable for quick results in medical diagnostics and drug research.

2 Widefield microscopes are available for our users. A Leica DMI8 Video-microscope and a Leica Thunder microscope. The Thunder is equiped with 8 Led sources of excitation:390,440,475,510,555,575,635,747 nm. Two Quad filters to spectrally separate the 8 channels on the following filters: 420-450; 462-484; 506-532; 527-551; 578-610; 602-680; 666-724; 770-850.

2 Leica SP8 confocal microscopes, equiped with HyD detectors and classical PMT detectors. The facility has expertise in practical training, counseling users in IF protocols, sample mounting and preparation.

We are equiped with one Nikon +Yokogawa W1 spinning disk, 6 wavelenghts. We propose technics like TIRF, FRET, FRAP and LiveSR (Gataca solution). The facility has strong expertise in dynamic high resolution imaging. Sample preparation for long time lapse imaging.

Strong expertise in sample preparation, transparisation, handling and imaging for Lightsheet microscopy

Confocal microscopy or/and spinning disk microscopy combined with sample expansion technique. Boyden and al.

STEDYCON Microscope Confocal and STED techniques all in one system. 775 STED laser + 405-488-560-640 lasers