Cellular imaging for neurodegenerative diseases studies : nervous system, neurons in culture, cell biology, cytoskeleton

Photomanipulation of biological objects (photoablation on spinning disks and on confocal equipped with 2photons, optogenetics)

The Spatial-Cell-ID facility aims to enhance the spatial resolution of MERFISH for pinpointing transcripts of specific genes in situ

Quantifying Physical parameters (rigidity, tension force production, turgor pressure…) from animal/plant cell to tissue (TFM, Brillouin imaging, photoablation, Triboindenteur, AFM, AFM Hybrid Stage)

Multiscale imaging of complex 3D objects: from embryonic to subcellular imaging (adaptive confocal and two-photon microscopy, light-sheet imaging of cleared tissues; NIR detection of luminescent probes, IA-based image analysis)

Multiscale measurements of molecular dynamics in cell and tissue (SPT, FCS, tICS, AiryScan, SIM, STED, SMLM, , Live SR, FRAP, live confocal imaging, Live TIRF and optogenetics)

We combine latest developments in cryo sample preparation and imaging by electron microscopies (cryo-FIB/SEM and cryo-TEM) to observe biomolecules of interest (ribosomes, carboxysomes, viruses, nuclear pore complexes…) within their cellular or tissular environment. Using cryo-electron tomography followed by image analysis, we are able to determine their 3D structures with near atomic resolution. Altogether, these methodologies pave the way for in situ structural biology studies.

Gene expression, DNA damage, DNA structure and DNA-binding proteins structures, super-resolution (SMLM, RIM, SIM, Airyscan), single particle tracking, photo-manipulation, Correlative Light Electron Microscopy (CLEM)

Atomic force microscopy on living cells or 3D samples is a powerful technique to measure the mechanical properties such as elastic moduli , rigidity, adhesion force, adhesion energy. Combining optical images, AI and AFM, cell population’s mechanome becomes accessible. 3D samples (tissues, organoids) mechanical properties require specific expertise we also provide.