Cell-biology expertise: providing cells(epithelial, glial, neuronal) and organoïds, labelling (transfections or probe-Test), and imaging for physicist, chemist that would like to test a prototype. Including : Guidance on super-resolution modalities (collaboration) : from system choice (SIM-STED-STORM) to images processing and data analysis (tools development).
Workflow: Sample preparation
:Service, Whole organ, Thick tissue cutting 500µm, Immuno-labeling
Expertise in preparation and morphological analysis using TEM (brain and spinal cord)
Full pipeline support on light sheet and biphoton imaging (from sample prep and tissue clearing to imaging)
Full pipeline support for single molecule RNA FISH
Rats, mice, non-human primates, mussels, shrimps, copepods
Phytotronic cabinets and expression of fluorescent fusion proteins/tags (transformation by electroporation)
Complete workflow from organic chemistry to fluorescence analysis (time-gated, TCSPC and MSC)
HPF, GP2, AFS2 Leica Microsystems
Confocal microscopy or/and spinning disk microscopy combined with sample expansion technique. Boyden and al.
Expertise in preparing biological samples using CUBIC transparisation, LS7 Zeiss light sheet microscopy imaging, and working with volumetric images (size reduction, segmentation).
Our 2 platforms offer routine services in histology, tissue processing, thin sections, staining, immunohistochemistry and digitisation of microscopy slides (Hamamtsu S360 & S60 scanners).