To develop the next generation imaging methods, we identified 5 strategic goals for instrumentation, and 2 accompanying goals for probe development and image processing (including deep learning).

Each goal is supervised by a dedicated Working Group (WG), which provides a national supervision for technological developments and implementations, as well as a forum for R&D exchanges, technology watch activities, and tools to transfer R&D innovations to facilities.

Strategic goal 1 – Super-resolution and single molecule tracking

  1. Develop new probes compatible with super-resolution microscopies and live cells to probe functional imaging at nanoscale
  2. Develop and implement new instrumentation to improve the resolution, allow in-depth multi-proteins nanoscopy and reveal new parameters at the nanoscale
  3. Implement computational approaches to tackle the issues that cannot be overcome physically or by traditional approaches

Strategic goal 2- Multiscale correlative microscopy

  1. Develop and test new probes, labels and sample preparations amenable to CLEM approaches
  2. Scout and Implement developments in CLEM
  3. Improve image processing for CLEM and 3D-EM

Strategic goal 3 – Multiscale light sheet microscopy (LSM)

  1. Develop multiphoton non-linear LSM to apply LSM on large specimen
  2. Develop technologies to improve LSM at high-speed and high-resolution
  3. Develop suitable image analysis and visualization tools

Strategic goal 4 – New contrast and in-depth imaging, preclinical microscopy

  1. Develop and implement label free microscopy modalities enabling new optical contrast
  2. Develop multimodal (label free, bright field staining and fluorescence) and automatisation (slide scanner, automatisation…) microscopy for histopathology
  3. Fill the gap between preclinical microscopy and medical imaging, by developing intravital microscopy, organoid screening and the correlation between living sample and 3D reconstructed fixed thick samples

Strategic goal 5 – High-content and multiplexed imaging

  1. Develop and implement smart microscopy techniques in FBI facilities
  2. Develop new methods to improve the throughput of current multiplexed technologies
  3. Combine multiplexed detection of RNA, chromatin and proteins with other imaging methods, leveraging machine learning / deep-learning approaches

Wanto to know more about our strategic goals? You can download France-BioImaging Roadmap 2023-2030 here.