[su_heading size=”24″]From Live cell imaging to Electron microscopy[/su_heading]
Institut Curie, Paris, France

• Topics: CryoCapsule, Live cell CLEM, High Pressure Freezing, Serial sectioning, Section CLEM, Registration
• Speaker and teachers: Xavier Heiligenstein, Ilse Hurbain, Claire Boulogne, Lucie Sengmanivong, Graca Raposo
• Registration: CLOSED
Correlative Light and Electron Microscopy (CLEM) takes an increasing place in scientific investigation. In our workshop, we propose hands-on training using our innovative protocol, centered around the CryoCapsule® and our newly developed software eC-CLEM®. Practical sessions will include cell culture on the CryoCapsule® and live cell imanging, High Pressure Freezing (HPM100 and HPM Light μ), Freeze Substitution to preserve fluorescence, serial sectioning, fluorescence on section, electron microscopy and image registration.
- It is firstly intended to scientists with advanced expertise in light microscopy and/or electron microscopy.
- 2 sessions of 4 people each will be organized to ensure that each participant gets the chance to practice.
- Evening discussions include presentation by each participant to have a change to discuss their own CLEM projects.
- A practical manual will be provided, covering all the steps of the method.

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in functional microscopy. These workshops are designed to complete various imaging devices: microscopes super resolution multiphoton, small animal imaging, FLIM / FCS CARS .. provided by the industrial partners of the school or by research teams in the case of prototype systems. Several workshops computer are also introduced for the analysis and processing of data. The basic rule of the School is that industrial undertake to allow full autonomy to scientific systems available. Piloting workshops for academic and not by industrial is indeed a key to the success of these practices phases.
