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Imagerie-Gif

Type: Facility

Business services: Yes

Head title: Head

Head name: Sandrine LECART

Contact: sandrine.lecart@i2BC.paris-saclay.fr

Website: https://www.i2bc.paris-saclay.fr/bioimaging/

Website Service: https://www.i2bc.paris-saclay.fr/bioimaging/

Node: Île de France Sud

Location: Plateforme Imagerie-gif, Avenue de la Terrasse, Gif-sur-Yvette, France

Available service offers on this site

High resolution volumic microscopy

The facility is equiped with a FIB-SEM (Focused Ion Beam-Scanning Electron Microscopy), that allows volumic acquisition of resin embedded samples at nanometric resolution (up to 5nm in 3D). This technology…
Electron microscopy

Liquid Liquid Phase Separation (LLPS) in vitro and in cellulo caracterization

The study of membrane-less compartmentalisation in cells is a growing field. At Imagerie-Gif, we have developed methods to identify these phenomena and quantify their dynamics using light microscopy techniques (DIC/FRAP/tracking,…
Molecular live imaging

Organelles and cell sorting (L1 and L2 environment) in Multisite

Identifying your cell or organelle populations according to morphology and/or fluorescence allows you to sort and purify them in tubes (from Eppendorf 1.5 ml to Falcon 50 ml) or plates…
Multiplex imaging and cytometry Others

Plant and insects genome size analysis by cytometry with automated detection

It is simple to measure genome size (pg DNA) of plants or insects with cytometry by isolating nuclei mechanically in adequate buffer.
Genomic imaging and nuclei visualization

Plant and insects genome size analysis by cytometry with automated detection

Genomic imaging and nuclei visualization

Protein and molecular dynamics imaging and analysis by FRAP, FRET-FLIM in cells

In order to characterise dynamic phenomena at the molecular level, we have developed expertise in evaluating their diffusion (FRAP) and highlighting interactions (FRAP/FRET-FLIM).
3D imaging Image analysis Intravital microscopy Molecular live imaging Multiplex imaging and cytometry Super-resolution microscopy

Quantitative imaging of bio or mecano-sensors using ratiometry, FLIM or cytometry approaches

Light microscopy enables biosensors to be used to characterize the cellular environment (pH, ROS, calcium, etc.) in order to highlight differences between populations or identify heterogeneities within tissues or cells…
Molecular live imaging Multiplex imaging and cytometry

Transmitted Electron Microscopy: from sample preparation to image analysis

Sample preparation is essential to observe biological samples in electron microscopy. Imagerie-Gif facility propose all the equipment to cover a large diversity of application: chemical fixation, high pressure freezing, resin…
Electron microscopy