Automated multi-position, phase contrast and fluorescence microscopy
Zeiss CD7, XY, Z-stacks, multi-points, mosaics, time-lapse
PRIMACEN
Type: Facility
Business services: Yes
Head title: Head
Head name: Ludovic GALAS
Contact: ludovic.galas@univ-rouen.fr
Website: https://primacen.crihan.fr/Site/index.php
Website Service: https://primacen.crihan.fr/Access/
Node: Normandie
Location: PRIMACEN, CURIB, Université de Rouen, Rue Lucien Tesnière, Mont-Saint-Aignan, France
Available service offers on this site
Cryo-confocal-FLIM microscopy
Stellaris 8, WLL, FALCON, Leica Microsystems
Development of image analysis solutions using artificial intelligence
Jupyter notebooks, visualisation and deep learning libraries (StarDist, CellPose, etc.)
FLIM-STED nanoscopy
Stellaris 8, WLL, FALCON, 592 and 775 nm for depletion, Leica Microsystems
High-pressure, automatic immersion freezing system, cryo-substitution automaton
HPF, GP2, AFS2 Leica Microsystems
Imaging for cardiovascular sciences
Workflow including sample preparation, light sheet microscopy, confocal microscopy, intravital microscopy, and image analysis.
Imaging for neurovascular sciences
Workflow including sample preparation, 2P microscopy, confocal microscopy, light sheet microscopy, ophthalmic imaging/OCT/ERG and image analysis.
Light sheet microscopy
Multidirectional illumination for transparent living samples (Alpha3 facility edition Phase View) and XXL chamber for transparent samples (Ultramicroscope Blaze, Milteniy BioTech)
Macro-Image J development and scripts
Groovy for Q-Path
Meso- and macro-imaging of marine invertebrates
Workflow including sample preparation, light sheet imaging, and fluorescence macroscopy
Metrology of photonic imaging systems
Numerous tests to monitor the performance of photonic microscopy systems, including light source power, observation field homogeneity, detector stability, etc. Training at PRIMACEN or at the applicants' site.
Microalgae imaging
Workflow from sample preparation, MET and cryo-confocal, advanced photonic microscopy WF/confocal/FLIM, CLEM, image analysis (AMIRA)
Most photonic microscopy in the L2 zone. L2 for animal/plant cell culture.
All equipment necessary for cell culture (PSM, incubators, centrifuges, etc.)
Multi-colour TIRF microscopy
IX83 microscope equipped with two PENTA SET TIRF, detector (sCMOS ORCA Fusion BT), TIRF module (445nm, 488nm, 514nm, 561nm, 640nm), cellVivo module, cellSens software (Evident)
RT and cryo transmission electron microscopy
Talos F200X G2
Straight spinning disk microscope for intravital imaging
Inscoper module for control, Nikon FNE stand, Yokogawa CSU W1 head, two cameras