SEBIO is a research unit specializing in aquatic ecotoxicology with a significant experience in the development and validation of effect-based tools for monitoring and predicting environmental impact of chemicals. As experts in marine biology, SEBIO develops in vivo whole aquatic organism imaging approaches aimed at achieving breakthrough in methods and physiological data in seawater shrimps, bivalves and fish larvae. By using light-sheet microscopy, multi-modal imaging and 3D-reconstruction, these projects will decipher fine structures and detailed homeostatic regulations, in particular related to vascular anatomy, immune cell migration and blood/hemolymph tissue perfusion, regarding infection disease, intertidal cycle, osmotic stress and blood withdrawal for laboratory sampling. In relation with INERIS, technological transfers to European environmental agencies and water monitoring programs will rely on a network of reference laboratories, research centers and organizations for environmental risk assessment.